) were produced by gynogenesis, and their growth and hereditary features were compared. The control group was a cross between a gynogenetic female and normal male. Twenty-four microsatellites markers were selected for the analysis. The results showed that 41, 55 and 78 alleles were scored in GI, GH and control group, respectively, with the average number of alleles as 1.7, 2.3 and 3.3. The average observed heterozygosity () among the 24 analyzed loci in the GH group was 0.654 9, which was higher than that in GI (0.419 2), but lower than that in the control group (0.916 7). The average homozygosity was 0.580 7 in GI and 0.345 1 in GH, which were much higher than that in the control group (0.083 3). The average similarity index among individuals in the GI group (0.831 2) was higher than that of the GH group (0.826 1), and both were higher than in the control group (0.6727). The growth rate of the GH group was significantly higher than the GI and control groups (<0.01). According to the result, GH lines have more advantages compared with GI lines and natural mating families(the control group). Hybridization between gynogenetic individuals could be an essential tool for breeding increased growth rates and to increase the genetic similarity of the Japanese flounder.