脊尾白虾抗细胞凋亡因子DAD1基因的克隆及其组织表达分析
DOI:
CSTR:
作者:
作者单位:

1. 农业部海洋渔业可持续发展重点实验室, 中国水产科学研究院 黄海水产研究所, 山东 青岛 266071;2. 中国水产科学研究院 南海水产研究所, 农业部渔业资源开发利用重点实验室, 广东 广州 510300

作者简介:

段亚飞(1989-), 男, 硕士研究生, 主要从事海水养殖生物种质资源与遗传育种研究. E-mail: dxfei87@126.com

通讯作者:

中图分类号:

Q959; S917

基金项目:

国家863计划项目(2012AA10A409); 国家虾产业技术体系项目(CARS-47); 公益性行业(农业)科研专项(201103034); 中央及公益性科研院所基本科研业务费项目(2013A0701); 山东省自主创新专项(2013CXC80202).


cDNA cloning and expression analysis of defender against cell death 1 (DAD1) gene in Exopalaemon carinicauda
Author:
Affiliation:

1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture; Yellow Sea Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Key laboratory of South China Sea Fishery Resources Exploitati

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    645 bp, 184 bp, 3′11412.85 kD, Penaeus monodon)与其他无脊椎动物的相似性为基因在脊尾白虾血细胞、鳃、肝胰腺、肌肉、卵巢、肠、胃和眼柄中均有表达(和EcDAD1),

    Abstract:

    Exopalaemon carinicauda, which was named is 645 bp, which contains a 184 bp 5′-UTR (untranslated region), 116 bp 3′-UTR, and 345 bp open reading frame (ORF) that encodes 114 amnio-acid polypeptides, which has the molecular mass of 12.85 kD and isoelectric point (pI) of 8.75. Homology analysis revealed that the amnio acid sequences of EcDAD1 had highly identifies with DAD1 of other species, for example, it had 92% identity with DAD1 of , and had 73%−80% identities with DAD1 of other invertebrates. The phylogenetic analysis showed that . The expression level of gene in different tissues were analyzed by quantitative real-time PCR. The results showed that hemocytes, gill, hepatopancreas, muscle, ovary, intestine, stomach and eye stalk, and the the highest expression level of gene was in ovary and white spot syndrome virus (WSSV), the relative expression level of might play an important role in the prawn immune system.

    参考文献
    相似文献
    引证文献
引用本文

段亚飞,刘萍,李吉涛,李健,高保全,陈萍.脊尾白虾抗细胞凋亡因子DAD1基因的克隆及其组织表达分析[J].中国水产科学,2014,21(1):44-52
DUAN Yafei, LIU Ping, LI Jitao, LI Jian, GAO Baoquan, CHEN Ping. cDNA cloning and expression analysis of defender against cell death 1 (DAD1) gene in Exopalaemon carinicauda[J]. Journal of Fishery Sciences of China,2014,21(1):44-52

复制
相关视频

分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2015-06-30
  • 出版日期:
文章二维码