Acipenser schrenckii) broodstock diets on reproductive performance and serum sex steroids levels were studied. Four groups of 48 female broodstocks, with an average body weight of (18.24±2.44) kg, were fed four artificial formulated diets containing 3.1%(SL1), 2.5%(SL2), 1.9%(SL3) and 1.3%(SL4) of n-3HUFA by adding different proportions of refined fish oil and corn oil for a period of 5 months from stage III of gonad development. Their reproductive performance, serum E₂ and T were evaluated. The results showed that after the Amur sturgeons were fed the diets for 5 months, their average body weight increased by 14.5%, while their average body weight dropped by 6.8% after winter and heating processing, with no significant difference between the trial groups (>0.05). Different n-3 HUFA and n-3 PUFA levels in feed had different effects on reproductive performance. The maturity rate and larval survival rate of the SL1 and SL2 groups were significantly higher than those of SL3 and SL4 group (<0.05), while the rates of abnormalities and deformities were significantly lower than that of the SL3 and SL4 group (<0.05). The diameter of the polarization egg of the SL1 group was significantly higher than that of the other groups (<0.05), while average fecundity was higher than other groups, but not significantly (>0.05). The fertilization rate and hatching rate were not significantly different among the trial groups ( level of each group was significantly <0.05), while the serum T level of each group decreased differently compared with before feeding (>0.05); however, no significant difference was found among the trial groups (> 0.05). This study indicated that the mixed oil is better than single fish oil or corn oil as a feed source of fat, the effect of fish oil is greater than that of corn oil in the feed at the same time, and the appropriate requirement forn-3 HUFA is approximately 2.5%.