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高温诱导对性成熟尼罗罗非鱼性腺中性别分化相关基因表达的影响
作者:
作者单位:

1. 山东农业大学 动物科技学院, 山东 泰安 271018;2. 山东省淡水水产研究所, 山东 济南250117

作者简介:

李春歌 (1987-), 女, 硕士, 从事水产动物遗传育种研究. E-mail: qwert_1235@163.com

中图分类号:

S965

基金项目:

中国博士后科学基金面上项目(20100481295); 山东省青年自然科学基金资助项目(ZR2009DQ021).


Differential expression analysis of genes involved in high-tempera­ture-induced sex differentiation in the gonads of mature Nile tilapia (Oreochromis niloticus)
Author:
Affiliation:

1. College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China; 2. Shandong Institute of Freshwater Fisheries, Jinan 250117, China

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    摘要:

    本研究通过分析高温诱导对性成熟尼罗罗非鱼性别分化相关基因表达的影响

    Abstract:

    In Nile tilapia, the mRNA expression level of various sex differentiation-related genes can be affected by high-temperature induction during the critical period of sexual differentiation, triggering sex-reversal from female to physiological male. However, it is unknown whether the effect of high temperature on Nile tilapia () sex differentiation-related genes is period-specific. In this study, the expression of eight sex differentiation-related genes, four heat shock protein genes and two methyltransferase genes were in 8-month-old Nile tilapia under high-temperature (36) treatment by quantitative real-time RT-PCR techniques. The results showed that the expression levels of , which are ovary development-related genes, were down-regulated (, which are testis development-related genes, were not significantly changed (>0.05) under high-temperature treatment. The results differed from those for high-temperature treatment of Nile tilapia during the critical period of sexual differentiation. The expression level of DNAJB1 was significantly down-regulated. The expression levels of were not significantly changed in the gonads of females and males under high-temperature induction. Furthermore, it was also found that high temperature significantly up-regulated the expression level of in the female gonad.

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李春歌,赵燕,陈红菊,付佩胜,王慧,季相山.高温诱导对性成熟尼罗罗非鱼性腺中性别分化相关基因表达的影响[J].中国水产科学,2014,21(2):214-224
LI Chunge, ZHAO Yan, CHEN Hongju, FU Peisheng, Wang Hui, JI Xiangshan. Differential expression analysis of genes involved in high-tempera­ture-induced sex differentiation in the gonads of mature Nile tilapia (Oreochromis niloticus)[J]. Journal of Fishery Sciences of China,2014,21(2):214-224

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