1. 上海海洋大学 农业部水产种质资源与养殖生态重点开放实验室 上海 201306;2. 上海市高校水产养殖学E-研究院, 上海海洋大学, 上海 201306
李倩, 硕士研究生, 从事水生动物细胞分子生物技术研究. E¬-mail: qianzai821@163.com
S968
国家自然科学基金资助项目(31201991); 上海市博士后科研资助计划面上项目(09R21413200); 教育部博士点基金项目(20123104120003); 上海市优秀青年项目(ssc11004); 上海市重点学科水生生物学建设项目(S30701); 上海市高校知识服务平台项目(ZF1206); 上海海洋大学博士启动基金资助项目.
1. Key Laboratory of Aquatic Genetic Research and Aquacultural Ecology Certificated by the Ministry of Agriculture, Shanghai 201306, China;2. Aquaculture Division, E-Institute of Shanghai Universities, Shanghai Ocean University, Shanghai 201306, China
Lea), 120 组标记细胞植入三角帆蚌外套膜中在蚌体内培养、小时运用, 迁出速度和细胞数量显著增加且细胞的活力随着培养时间的延长而逐渐增大时后显著下降但在活体培养
In China, is the shellfish of choice for cultivating freshwater pearls; however, there has been very little research on cell and mantle tissue culture. Based on DMEM), this study attempted to optimize modified formula of medium and buffer for mantle tissue and cell culture. The ratio of RNA/DNA as an evaluation index of cell proliferation was determined and, using microscopic observations, tissue culture cells versus time, speed and cell energy were established. Hoechst fluorescent staining of DNA was carried out using 24-, 108- and 120-h cells of culture, and then the three marked cell groups were implanted into the mantle for culture . fter optimization, the buffer and medium improved cell migration from the mantle, the velocity of migration and the significantly increased cell number (<0.05).Cell vitality also increased with incubation time. Cell vitality was maximal for culture at 108 h, with a RNA/DNA ratio of 24.53, but was followed by a significant decline (<0.05).Microscopic observations of cells were in agreement with the determined RNA/DNA index values. When in vitro activity improved and culture conditions increased cell activity, with RNA/DNA values up to 25.45, but vitality declined significantly after 168 h when injected into the cell for activity of cells, it would appear that a living environment has no significant effect on cell viability. Our study provides basic but valuable information for further research on mantle cell proliferation and the establishment of cell lines
李倩,施志仪,李文娟,黄凯,祁晓翔.三角帆蚌外套膜细胞体外培养优化及体内植入培养对细胞生长的影响[J].中国水产科学,2014,21(2):225-234
LI Qian, SHI Zhiyi, LI Wenjuan, HUANG Kai, QI Xiaoxiang. Impact of in vitro optimization and in vivo implantation culture on the growth of Hyriopsis cumingii mantle cells[J]. Journal of Fishery Sciences of China,2014,21(2):225-234
