, is an important genetic resource. To preserve the species, experimental trials were carried out to find suitable sperm cryopreservation methods by comparing the effects of four extenders, D-15, D-17, Ringer’s and M-Hank’s, and five cryoprotectants, dimethyl sulfoxide (DMSO), glycerol (Gly), ethylene glycol (EG), propylene glycol (PG) and dimethylformamide (DMF), on sperm motility after thawing. The present study demonstrates that DMSO and D-17 are a suitable choice as cryoprotectant and extender, respectively. Using a protocol of diluting a sperm sample with D-17 at a sperm/extender ratio of 13, adding DMSO to a final concentration of 10% in volume, loading to 2-mL cryotubes as containers, following the three-step method to cool the mixture and thawing the frozen semen in a 37 water bath for 80 s, a post-thaw motility of >80% could be obtained using computer-assisted sperm analysis (CASA). Single cell gel electrophoresis showed that >70% of sperm DNA was intact and practically no sperm nuclear DNA was severely damaged. Flow cytometric analysis showed that 26.74% of the frozen-thawed sperm had intact membrane and functional mitochondria. The highest fertilization rate was (39.6±6.5)% in the hybridization of (♂). The hatching time was 38 h after fertilization at 24–28 and body parameters after being fed for 7 day were (0.045±0.020) g in weight, (1.346±0.255) cm in length and (0.438±0.103) cm in depth. Thus, it is recommended that D-17+10% DMSO be used for cryopreservation of S. scherzeri germplasm resources and promote crossbreeding with (♀) through the successful application of sperm cryopreservation skills.