and non-ionic ammonia stresses on the apoptosis of cultured in freshwater, changes of cyt-C content and caspase-3 activity were determined by enzyme-linked immunosorbent assay (ELISA) methods after acute temperature variation (16℃←22℃→28℃) and non-ionic ammonia (NH₃), and compared with those of seawater shrimps. It was found that: (1) Thermal stresses significantly increased the cyt-C content in heamolymph and hepatopancreas of both freshwater and seawater shrimps within 5 d (<0.05); three-way ANOVA analysis results showed that culture condition, thermal stress and exposure time all had a significant impact on cyt-C content in shrimps (>0.05). (2) In freshwater shrimps, the caspase-3 activity in heamolymph and hepatopancreas changed significantly after thermal stresses, and increased significantly at 5 d compared to that before experiment (0 d) (<0.05), except the heamolymph after hyperthermal stress, which was different to those in seawater shrimps; culture condition, thermal stress and exposure time all had a significant impact on caspase-3 activity of shrimps and the interaction among them was significant ( concentration of NH₃N, the cyt-C content of heamolymph and hepatopancreas increased significantly in both freshwater and seawater groups ( concentration, cyt-C content in freshwater and seawater shrimps showed a similar change trend: cyt-C content increased significantly within 2N stress and exposure time significantly affected cyt-C content but only cyt-C content in hepatopancreas was significantly affected by culture condition and interaction among them was significant(<0.05). (4) Change trend of caspase-3 activity in heamolymph and hepatopancreas of freshwater and seawater shrimps were similar with those of cyt-C contents, only except hepatopancreas caspase-3 activity kept rising; culture condition, NH₃N stress and exposure time significantly affected caspase-3 activity and caspase-3 activity in heamolymph was significantly affected by their interaction ( in freshwater had a worse adaptive capacity than the ones in seawater under hypothermal stress; the in freshwater showed a similar change of cytthat in seawater in response to NH₃N stress could significantly affect cyt-C content and caspase-3 activity in heamolymph and hepatopancreas of N concentration and avert that water temperature fluctuate dramatically especially in freshwater culture.