复合水产养殖环境中气单胞菌耐药性及其同源性分析
作者单位:

1. 中国水产科学研究院 珠江水产研究所, 农业部渔用药物创制重点实验室, 广东省免疫技术重点实验室, 广东 广州, 510380; 2. 上海海洋大学 水产与生命学院, 上海 201306

作者简介:

黄玉萍(1988–), 女, 硕士研究生. E-mail: hyp.ch@163.com

中图分类号:

S94

基金项目:

国家自然科学基金青年项目(31302228); 广东省自然科学基金博士启动项目(S2013040013070); 公益性行业(农业)科研专项(201203085).


Antimicrobial resistance and homology analysis in Aeromonas isolated from integrated fish farms
Author:
Affiliation:

1. Key Laboratory of Fishery Drug Development, Ministry of Agriculture; Key Laboratory of Immune Technology of Guangdong Province; Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China 2. College of Fisheri

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    摘要:

    个不同畜禽/共测定其对种药物的敏感性进行肠杆菌基因间重复序列株气单胞菌对氨苄西林、阿莫西林PFGE个簇。两种分型方法均发现来源于同一养殖场的菌株存在相同或相似图谱的分离株提示此为同一克隆株。实验结果表明本研究通过对不同来源气单胞菌进行耐药性分析及

    Abstract:

    Integrated fish farming is commonly practiced in Guangdong Province in South China. Animal manure generated from livestock and poultry (commonly pigs and ducks) is directly excreted into, or transported via pipes, to fish ponds where the nutrients support the growth of photosynthetic organisms. The use of a wide variety of antimicrobials in livestock and poultry has led to antimicrobial-resistant bacteria. Thus, there is concern over the potential for transfer of these bacteria into the integrated culture systems. Our objective was to determine the antimicrobial susceptibility and homology of were isolated from pig/duck waste, fish, pond water, and sediments from four integrated fish farms in Guangdong Province. We used the Kirby-Bauer disk diffusion method to determine the susceptibilities to 8 classes of 24 antimicrobials. The genomic DNA of the isolates was extracted and the molecular typing was analyzed by entero bacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) and pulsed field gel electrophoresis (PFGE).The resistance frequencies of ampicillin, amoxicillin/clavulanate, and rifampicin were relatively high in the 57 isolates, and the resistance profiles varied among the different isolates. The isolates were grouped into 24 clusters by ERIC-PCR and 46 clusters by PFGE. Isolates from different sources within the same integrated fish farm exhibited similar or identical genotyping patterns between the two molecular typing methods, and they were also associated with similar resistance patterns suggesting they were homologous. Our results suggest that ERIC-PCR and PFGE have use for analysis of clonal relationships and tracing of the source of resistance in Integrated fish farming systems appear to be susceptible to the transfer of drug-resistant bacterial strains from livestock and poultry to fish or the environment. The impact of antimicrobial resistance in the fish was not addressed in this study and remains to be determined.

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黄玉萍,邓玉婷,姜兰,谭爱萍,吴雅丽,王伟利,罗理.复合水产养殖环境中气单胞菌耐药性及其同源性分析[J].中国水产科学,2014,21(4):777-785
AHUANG Yuping, DENG Yuting, JIANG Lan, TAN Aiping, WU Yali, WANG Weili, LUO Lieromonas, integrated fish farm, antimicrobial resistance, ERIC-PCR, PFGE, clonal transmission. Antimicrobial resistance and homology analysis in Aeromonas isolated from integrated fish farms[J]. Journal of Fishery Sciences of China,2014,21(4):777-785

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