A two-factorial experiment was designed to study the effects of temperature (10, 15, 20, and 25–^{Gracilaria chouae. And the determination of the relative growth rate, photosynthetic pigment content, malondialdehyde (MDA) and superoxide dismutase (SOD) content and other indicators were carried out. The results showed that temperature, light intensity, and their interactions had significant effects on the growth of andthe effects of temperature was greater than those of light intensity. A higher growth rate of and 20·d–1. both temperature and light intensity had significant effects on the In general, the photosynthetic pigment contents of increased with the rise of temperature, while it increased initially and then dropped down with the rise of light intensity. The contents of phycoerythrin (PE), phycocyanin (PC), allophycocyanin (APC), and chlorophyll a were 1.12 mg/g, 0.272 mg/g, 0.216 mg/g and 1.82mg/g separately when the light intensity was 120 μmol·m–2·s–1 and temperature was 25Temperature, light intensity, and their interactions had significant effects on the SOD and MDA contents of And the effect of temperature on the content of MDA was greater than that of light intensity. The MDA content of increased initially and then dropped down with increating light intensity and temperature.The content of SOD increased with increasing temperature and light intensity. And light intensity was the main factor affecting the SOD content of}