鳗鲡病原性嗜水气单胞菌与爱德华氏菌二联表达外膜蛋白的免疫原性

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鳗鲡病原性嗜水气单胞菌与爱德华氏菌二联表达外膜蛋白的免疫原性
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作者单位:集美大学 水产学院 鳗鲡现代产业技术教育部工程研究中心, 福建 厦门 361021
作者简介:郭松林(1976–), 男, 博士, 副教授, 从事鱼类病原与免疫防治研究. E-mail: gsl@jmu.edu.cn
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中图分类号:S942
基金项目:国家自然科学基金项目(31001136).

Immunogenicity of a bivalently-expressed outer membrane protein of Aeromonas hydrophila and Edwardsiella tarda from diseased eels

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Fishery College, Anguilla Modern Engineering Research Center of Industrial Technology of the Ministry of Education, Jimei University, Xiamen 361021, China

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Aeromonas hydrophila))尾日本鳗鲡平均分为PBS(0.01 mol/L, pH7.4)CFU /mL)腹腔注射、麻醉鳗鲡采血并分离抗凝血。测定个时间点鳗鲡全血细胞的转化水平。免疫后3灭活菌和外膜蛋白免疫组的抗体水平均极显著高于<0.01)不同处理组不同时间段血清、黏液和肝肾组织悬液的溶菌酶含量存在显著P<0.01)<0.05), <0.05)PBS。本研究结果表明鳗鲡源嗜水气单胞菌和迟缓爱德华氏菌外膜蛋白二联表达产物免疫日本鳗鲡后可提高鳗鲡的免疫功能及其对这两株菌的抵抗力

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) were evaluated after they were immunized with a bivalently-expressed outer membrane protein (OMP) of porin II and ompS2 from , respectively. One-hundred and fifty eels distributed into three equal groups were administered ) injections ofA. hydrophila and The plasma collected on days 14, 21 and 28 were used to measure the titers of specific antibodies and lysozyme activity, and whole blood cells collected on these days were used to evaluate the stimulation index (SI). Lysozyme activities in skin mucus and suspensions of liver and kidney were also recorded on these days. On day 28 post-immunization, eels from all three groups were challenged by . Compared with the PBS group, the serum titers of the anti-<0.01) on days 21 and 28. Compared with the PBS and OMP groups, proliferation of whole blood cells in the FKC group was significantly (<0.05) enhanced on day 14, but whole blood cell proliferation in the two immunized groups significantly (<0.05) decreased on day 21 compared with the PBS group. Lysozyme activity in serum, skin mucus, and the liver and kidney suspensions differed significantly (<0.01) between the three groups. The relative percentage survival after challenge with <0.05). These results show that bivalent OMP improved the immune function of the Japanese eel and protected it against infection with . This vaccine has potential for use in freshwater farmed eels.

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郭松林,冯建军,陆盼盼,曹伟棋,赵金平.鳗鲡病原性嗜水气单胞菌与爱德华氏菌二联表达外膜蛋白的免疫原性[J].中国水产科学,2015,22(1):113-120
GUO Songlin, FENG Jianjun, LU Panpan, CAO Weiqi, ZHAO Jinping. Immunogenicity of a bivalently-expressed outer membrane protein of Aeromonas hydrophila and Edwardsiella tarda from diseased eels[J]. Journal of Fishery Sciences of China,2015,22(1):113-120

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在线发布日期: 2015-06-23
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