1. 中国科学院 水生生物研究所, 淡水生态与生物技术国家重点实验室, 湖北 武汉430072; 2. 中国科学院大学, 北京 100049
李涛 (1992–), 男, 研究生, 现正从事水生病毒分子生物学研究. E-mail: 469445704@qq.com
S94
国家科技支撑计划课题 (2012BAD25B02).
1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China
Andriasda vidianus 这是一个经生物信息分析表明与病毒感染相关的功能基因。先经扩增长到的pMD18-T-6R pET-32a-6R, , 其编码分子量约为、含个氨基酸的蛋白与水产动物病毒蛋白家族进行转化、诱导表达获得与预测分子量相符的融合蛋白
gene was. Then, the recombinant prokaryotic expression plasmid pET-32a-6R was constructed and sequenced. ADRV gene was blasted with homologous sequences from other rana viruses of aquatic animals. The gene encodes a protein of 236aa with a predicted molecular mass of 27 kD. Amino acid sequence alignment of ADRV members in the viruses-6R was transformed into DE3 and induced by IPTG. The expressed protein was purified with Ni-NTA His-Bind affinity chromatography, separated by different concentrations of imidazole eluent and analyzed by SDS-PAGE. A 45 kD fusion proteincontaining protein and 18 kD his-tagged was obtained in accordance with the predicted molecular weight. These results provide useful experimental material for antibody preparation and study on ADRV-host interactions.
李涛,陈中元,张奇亚.一个与大鲵蛙病毒 (ADRV) 感染相关基因 —6R的克隆、原核表达及其产物分离[J].中国水产科学,2015,22(2):353-358
LI Tao, CHEN Zhongyuan, ZHANG Qiya. Cloning, prokaryotic expression and protein separation of 6R, an Andrias davidianus ranaviurs (ADRV) infection-related gene[J]. Journal of Fishery Sciences of China,2015,22(2):353-358
