parents, released fish and recapturedfish DNA, and obtained the mtDNA control areas sequences of these groups. The results showed that 55 parents had 26kinds of haplotypes; and the 129 released fish had 5 kinds of haplotypes, all corresponding to the parents haplotypesdatabase, verified the accuracy of mtDNA markers in the anaylsis of paretal allocation for the released with 70 kinds of haplotypes were identified, amongwhich 330 fish with 17 kinds of haplotypes were corresponded to the parents haplotypes database. These fish had thepossibility to be the released fish. Another 105 fishes with 53 kinds of haplotypes could not be corresponded to the parents’haplotypes database, and we categorized them into non-released fish of Beidaihe. We performed further paternitytests by using 4 high polymorphic microsatellite markers for the 330 might be released ’’ alleles, and were determined as non-released. Bythe combination using of mtDNA and microsatellite markers, we can categorized 310 fish (among 435 recaptured ). In conclusion, we could effectively determine the released from the recaptured ones, and provide basic information for the analyzing of genetic diversity and artificial stock enhancementeffect.