(Perciformes, Odontobutidae), is a small demersal freshwatergoby and has recently been considered a promising candidate for aquaculture in China. However, until nowthere has been limited genetic information regarding was obtained by primer-walking PCR amplification, and the mtDNA lengthwas 16846 bp. Then, mtDNA structure, gene rearrangement mechanism, and application in phylogenetic reconstructionwere analyzed. The mtDNA of contained 37 genes (13 protein-coding genes, two rRNAs,and 22 tRNAs) and non-coding control regions. In addition to Gln), all other components were encoded on the heavy strand. Allprotein-coding genes initiated with the typical ATG sequence except CO I (with GTG) and (with ATA).These 13 protein-coding genes had TAA, TA−, and T− as termination codons. Because of shuffling rearrangementof different tRNAs, the classic mtDNA arrangement HSL (tRNA). Consequently, 320 bp and 42 bp anonymous regions were inserted between tRNAand tRNA, respectively. However, the content of A+T (55.3%) was higher thanthat of G+C (46.7%), and it was similar to other fish mtDNA. Among the 112 analyzed Perciforme fish species,only 13 (11.61%) experienced mtDNA gene rearrangement. In particular, the gene rearrangement of (KF154120), which indicatesthat this is an important molecular ‘tag’ for the evolution of the . Selection pressure and geneticdiversity analyses of the 13 protein-coding genes revealed that might be suitable molecular candidatemarkers for reconstructing phylogenetic relationships in the Gobioidei family. In the ML phylogenetic tree for10 species of Gobioidei based on