, germplasm, it is urgent for the pearl industry toselect varieties with optimal traits. Analysis of genotype and environment interactions is important for verifyingand promoting elite varieties, for selecting breeding varieties suited to specific environments, and for determiningbreeding objectives. In this study, we selected three typical families (F, and F) with fast, median, and slowgrowth that were cultured in Li’an (Lingshui, Hainan), Tandou (Zhanjiang, Guangdong), and Bailong (Fangcheng,Guangxi) for 1 year. Eight hundred individuals per family were cultured in each location. The three familie lineswere started in January, 2012. Multiple analysis of variance (ANOVA) and additive main effects and multiplicativeinteractions (AMMI) were used to analyze the effects of genotype-by-environment (G×E) interaction on differentgrowth stages. Results showed that the growth rate of different generation had the fastest growth rate with regard to total weight, whereasF2 had the fastest growth rate with regard to shell length, shell height, and shell width. Intotal, the proportions of genotypic(G), environmental(E), and G×E interaction variances were 72.47%–88.55%,9%–24.13%, and 3.32%–4.04% for the four traits, respectively. In different growth stages, genotypic variance reducedfrom 92.32%–98.66% to 32.45%–68.10%. Instead, environmental variance increased from 0.11%–1.27% to24.41%–62.26%, and G×E variance increased from 0.79%–3.9% to 3.84%–7.51%. Analysis of AMMI biplots offour traits showed that F2 was the most stablefamily. Above all, the effect of G×E interaction was small. Family greatly contributed to phenotypic variation. Ourobservations provide support for selective breeding of this animal.