The purpose of this research was to study the bacterial community structure in digestive tract and enzymeproduction capacity of enzyme-producing bacteria, and provide reference for selection and application ofprobiotics for carnivorous fish culture. In this experiment, samples of juvenile saladfish ()stomach, pyloric caeca, foregut, midgut, and hindgut were obtained in recirculating aquaculture systems. Bacterialcommunity structure was analyzed using 16S rDNA-PCR. The enzyme-producing bacteria were isolated and identifiedby isolating and screening enzyme-producing bacteria. Moreover, the enzyme activities were tested.Twenty-seven strains were isolated and cultured under experimental conditions, including 13 strains of , which accounted for 48.2%, 18.5%, 25.9%, 3.7%, and 3.7%, respectively, of the isolated bacteria.The sequence homology of corresponding genes was greater than 98%. Fifteen strains produced enzymes and accountedfor 55.6% of all bacteria; these bacteria included 7 strains of . Among these bacteria, 13 strains can produce bothprotease and amylase, whereas 4 strains can produce protease, amylase, and lipase. Among the enzyme-producingbacteria, 5 strains can produce 3 enzymes and 9 strains can produce 2 enzymes. Moreover, the bacteria in themidgut and hindgut were most abundant, and those in the stomach, diverticulum pyloricum and foregut were lessabundant; the bacteria that produce lipase were concentrated in the midgut. Protease and amylase were the mainenzymes produced by these bacteria; these two enzymes were highly productive, with protease activity up to(87.7321.134) U/mL and amylase activity between (77.176±0.599) U/mL and (73.458±0.574) U/mL. Only onestrain produced cellulase, and the activity was low. Under the experimental conditions, the isolated bacteria wereall culturable. However, non-culturable bacteria cannot be isolated. Moreover, some culturable bacteria in the digestivetract could not be isolated because of limited testing conditions such as temperature, pH, culture medium,and other factors that may affect normal bacteria growth. In addition, isolation and identification took place underaerobic conditions, which is not similar to real gut conditions; thus, a large number of anaerobic bacteria were notisolated. Therefore, further investigation is needed to determine the actual bacterial community structure of the digestive tract. Our data showed that the bacterial community structure of the digestive tract directly affectedthe activity and diversity of exogenous digestive enzymes. This research provides a theoretical basis forselection of enzyme-producing bacteria in recirculating aquaculture conditions.