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黄斑蓝子鱼LC-PUFA 合成代谢与渗透压调节的关系研究
作者:
作者单位:

1. 汕头大学 海洋生物研究所 广东省海洋生物技术重点实验室, 广东 汕头 515063; 2. 河南师范大学 水产学院, 河南 新乡 453007; 3. 广东溢多利生物科技股份有限公司, 广东 珠海 519060

作者简介:

作者简介: 谢帝芝(1986−), 男, 博士, 讲师, 研究方向为水产动物营养与饲料. E-mail: xiedizhi@qq.com 通信作者: 李远友, 教授, 博士生导师. E-mail: yyli@stu.edu.cn

中图分类号:

S963

基金项目:

国家自然科学基金重大国际合作研究项目(31110103913); 国家自然科学基金面上项目(41276179); 国家自然科学基金青年科学基金项目(31202012, 31202011).


Research on relationship between LC-PUFA biosynthesis andosmoregulation in Siganus canaliculatus
Author:
  • XIE Dizhi1

    XIE Dizhi1

    1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou 515063, China; 2. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai 5190
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    2

    1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou 515063, China; 2. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai 5190
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  • XU Shude1

    XU Shude1

    1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou 515063, China; 2. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai 5190
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  • 3

    3

    1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou 515063, China; 2. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai 5190
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  • CHEN Fang1

    CHEN Fang1

    1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou 515063, China; 2. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai 5190
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  • WANG Shuqi1

    WANG Shuqi1

    1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou 515063, China; 2. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai 5190
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  • YOU Cuihong1

    YOU Cuihong1

    1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou 515063, China; 2. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai 5190
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  • LI Yuanyou1

    LI Yuanyou1

    1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou 515063, China; 2. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai 5190
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Affiliation:

1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Marine Biology Institute, Shantou University, Shantou 515063, China; 2. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 3. Guangdong VTR Bio-tech Co., Ltd., Zhuhai 5190

  • 摘要
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    摘要:

    为探讨黄斑蓝子鱼(Siganus canaliculatus)长链多不饱和脂肪酸(long-chain polyunsaturated fatty acids,LC-PUFA)合成代谢与渗透压调节的关系, 本研究以鱼油(FO)和混合植物油(苏子油与双低菜籽油, VO)为脂肪源配制两种等氮等脂饲料, 投喂饲养在3 种盐度(10、20 和32)下的黄斑蓝子鱼幼鱼8 周后, 分析了各处理组幼鱼的生长性能和鳃的磷脂脂肪酸组成、Na+/K+-ATPase (NKA)活力及其基因表达。结果显示, 相同盐度下, VO 组和FO 组鱼的生长性能差异不显著(P>0.05); FO 组鱼鳃磷脂中的n-3 LC-PUFA 含量显著高于VO 组(P<0.05), 但VO 组鱼的n-6 LC-PUFA水平显著高于FO组(P<0.05); VO 组鱼鳃的NKA酶活力及其mRNA表达量都显著高于FO组(P<0.05)。不同盐度下, 无论VO 组还是FO 组的鱼, 盐度10 组鱼的生长性能显著低于盐度20 和32 组(P<0.05), 而其鳃的LC-PUFA 含量、NKA 酶活力及其mRNA 表达量都显著高于盐度20 和32 组(P<0.05), 各指标在后两个盐度组之间差异不显著(P>0.05)。由此可见, 盐度10 对黄斑蓝子鱼具有一定的胁迫性, 导致其生长性能较差。摄食鱼油脂肪源饲料, 可以提高鱼鳃磷脂的n-3 LC-PUFA 水平; 而摄食植物脂肪源饲料时, 鱼体可能通过自身合成的n-6LC-PUFA 调控鳃的NKA 基因表达及其酶活力以调节渗透压。

    Abstract:

    To explore the relationship between osmoregulation and long-chain polyunsaturated fatty acid(LC-PUFA) biosynthesis in rabbitfish (), an 8-week feeding trial was performed in juvenilerabbitfish with diets that contained fish oil (FO) or a blend of vegetable oils (VO; perilla and canola oils) at threesalinities: 10, 20, and 32. The growth performance, fatty acid composition of gill phospholipids, and activity andexpression levels of gill NaATPase (NKA) under different treatments were analyzed. The results showed that,at the same salinity, there was no effect of dietary lipid source on growth performance (0.05), whereas n-3LC-PUFA content in gill phospholipids in the FO dietary groups was significantly higher than those in VO treatments(0.05). In particular, although the contents of n-6 LC-PUFA in VO-fed fish were lower than in FO-fedfish, the opposite was observed for n-6 LC-PUFA contents. Furthermore, the activity and mRNA expression levelsof gill NKA in VO groups were significantly higher than those in FO groups (0.05). Under different salinities,the growth indexes at a salinity of 10 were significantly lower than those at salinities of 20 and 32 in both dietarygroups (0.05), whereas the LC-PUFA contents, and activity and mRNA expression levels of NKA in fish fedVO were significantly higher than that in fish fed VO (0.05). In addition, there were no significant differencesin either of the higher two salinities treatments (0.05). The results indicate that a salinity of 10 may not suitablefor rabbitfish culture. Furthermore, FO-derived diets can improve n-3 LC-PUFA content in gill phospholipids,which helps maintain membrane lipid fluidity and osmotic balance. Alternatively, VO-derived diets can regulateosmotic pressure by synthesizing n-6 LC-PUFA to up-regulate NKA expression and activity.

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谢帝芝,徐树德,陈芳,王树启,游翠红,李远友.黄斑蓝子鱼LC-PUFA 合成代谢与渗透压调节的关系研究[J].中国水产科学,2015,22(5):950-959
XIE Dizhi, XU Shude, CHEN Fang, WANG Shuqi, YOU Cuihong, LI Yuanyou. Research on relationship between LC-PUFA biosynthesis andosmoregulation in Siganus canaliculatus[J]. Journal of Fishery Sciences of China,2015,22(5):950-959

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