嗜水气单胞菌在浸泡感染团头鲂的组织动态分布
作者:
作者单位:

1. 南京农业大学 无锡渔业学院, 江苏 无锡 214081; 2. 中国水产科学研究院 淡水渔业研究中心, 农业部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081

作者简介:

作者简介: 陆春云(1987–), 男, 硕士研究生, 主要研究方向为水产动物疾病与营养. E-mail: luchunyun1987@sina.cn 通信作者: 谢骏, 研究员. E-mail: xiej@ffrc.cn; 习丙文, 副研究员. E-mail: xibw@ffrc.cn

中图分类号:

S941

基金项目:

现代农业产业技术体系建设专项资金项目(CARS-46-10).


Infection kinetics of Aeromonas hydrophila in the blunt-snout bream,Megalobrama amblycephala
Author:
  • LU Chunyun1

    LU Chunyun1

    1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery S
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    2

    1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery S
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  • XIE Jun1

    XIE Jun1

    1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery S
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  • XI Bingwen2

    XI Bingwen2

    1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery S
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  • LIANG Liguo2

    LIANG Liguo2

    1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery S
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  • XIA Fei2

    XIA Fei2

    1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery S
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  • PAN Liangkun2

    PAN Liangkun2

    1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery S
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Affiliation:

1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery S

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    摘要:

    探究温度对浸泡感染后嗜水气单胞菌在团头鲂各组织分布的影响。实验设立水温组℃水温其中、试验组攻毒后分别于、、, 在各取样时间段实验组 和实验组 团头鲂各组织在均能检测到荧光嗜水气单胞菌最高菌量出现在鳃(其次是脾、肾组织内的菌量随时间大体呈现先上升后下降的趋势实验组中各组织菌量显著大于。研究结果表明, ℃

    Abstract:

    Fish disease has become an increasingly prominent problem in aquaculture development and causessevere economic damage to fisheries. is an important fish pathogen that causes hemorrhagingand septicemia in many cultured fishes. The infection kinetics of WJ-8, that was transformed with aplasmid encoding green fluorescent protein (pEGFPuv-Kar). The purpose of this investigation was to fully elucidatethe pathogenesis of septicemia in blunt-snout bream and provide basic data on pathogenic factors of .Fish were divided into three experimental groups: group A, water temperature 25℃GFP μfor 24 h, and bacteria was counted and then transferred to a fish culture tank at afinal concentration of 10CFU/mL. The bacteria bath challenge was maintained for 30 min for groups A and B butnot for group C. Then, fish were transferred to tanks with clean water and the original water temperature wasmaintained. The bacterial counts from the blood, spleen, kidney, gills, intestine, and muscle were detected at 2 h, 4h, 8 h, 12 h, and 24 h after challenge. Bacteria were detected in both infected groups (A and B) but not in the uninfectedcontrol group (C) at all times during the experiment. An increased number of bacteria was observed in thegills of fish in groups A and B (0.05), whereas large numbers of bacteria were also noted in the spleen, kidney,and intestine. There were more bacteria that attached to and proliferated on the fish gills at 32. Thebacterial counts for group B were significantly higher than those of groups A and C (in blunt-snout bream, and high watertemperature is associated with heightened infection risk.

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陆春云,谢骏,习丙文,梁利国,夏飞,潘良坤.嗜水气单胞菌在浸泡感染团头鲂的组织动态分布[J].中国水产科学,2015,22(5):1068-1074
LU Chunyun, XIE Jun, XI Bingwen, LIANG Liguo, XIA Fei, PAN Liangkun. Infection kinetics of Aeromonas hydrophila in the blunt-snout bream, Megalobrama amblycephala[J]. Journal of Fishery Sciences of China,2015,22(5):1068-1074

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  • 在线发布日期: 2015-09-16
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