吉富罗非鱼对饲料亚油酸的需要量
作者:
作者单位:

1. 中国海洋大学 农业部水产动物营养与饲料重点实验室, 山东 青岛 266003; 2. 中国水产科学研究院 长江水产研究所, 农业部淡水生物多样性保护与利用重点开放实验室, 湖北 武汉 430223

作者简介:

作者简介: 田娟(1983–), 女, 博士研究生, 主要从事鱼类营养学研究. E-mail: tianjuan0303@163.com 通信作者: 文华, 博士, 研究员, 主要从事鱼类营养与饲料研究. E-mail: wenhua.hb@163.com

中图分类号:

S963

基金项目:

现代农业产业技术体系建设专项资金项目(CARS-49); 农业部公益性行业科研专项经费项目(201003020).


Optimal dietary linoleic acid requirement for the advanced juvenile GIFT strain of Nile tilapia, Oreochromis niloticus 
Author:
  • TIAN Juan1

    TIAN Juan1

    1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins
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    1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins
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  • TU Wei2

    TU Wei2

    1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins
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  • WEN Hua2

    WEN Hua2

    1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins
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  • JIANG Ming2

    JIANG Ming2

    1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins
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  • WU Fan2

    WU Fan2

    1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins
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  • LIU Wei2

    LIU Wei2

    1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins
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  • YANG Changgeng2

    YANG Changgeng2

    1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins
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  • HUANG Feng2

    HUANG Feng2

    1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins
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Affiliation:

1. Key Laboratory of Aquaculture Nutrition, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China; 2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture; Yangtze River Fisheries Research Ins

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    摘要:

    选用初始体重为(60.98±3.82) g 的吉富罗非鱼(Oreochromis niloticus)630 尾, 随机分成7 组(每组设3 个重复,每个重复30 尾), 饲喂亚油酸含量分别为0.07%(对照组)、0.36%、0.61%、1.03%、2.00%、3.00%和4.15%的7 种半纯化等能等氮饲料10 周。结果表明, 鱼体增重率、饲料效率、蛋白质效率和蛋白质沉积率均在亚油酸水平为1.03%时较对照组差异显著(P<0.05), 且均在饲料亚油酸水平为2.00%时达到最大。经二次回归分析, 饲料亚油酸水平为2.49%和2.66%时吉富罗非鱼分别获得最大增重率和最高饲料效率。通过折线回归发现饲料亚油酸水平为1.02%时, 吉富罗非鱼获得最大蛋白沉积。肝体比和脏体比均随亚油酸水平的升高而升高, 当饲料亚油酸含量为0.61%~4.15%时显著高于对照组(P<0.05)。亚油酸添加组的肌肉粗脂肪含量显著高于对照组(P<0.05); 当饲料亚油酸含量为1.03%~4.15%时, 肝和全鱼粗脂肪含量显著高于对照组(P<0.05)。随饲料亚油酸水平升高, 血清甘油三酯和总胆固醇变化趋势一致, 呈现先下降后上升的趋势, 经二次回归分析亚油酸水平为1.63%时血清甘油三酯含量最低; 各亚油酸添加组的血清高密度脂蛋白胆固醇含量显著高于对照组, 且在饲料亚油酸含量为1.13%时达到最大(P<0.05); 当饲料亚油酸水平为1.03%~4.15%时, 血清低密度脂蛋白胆固醇含量显著低于对照组(P<0.05)。随饲料中饱和脂肪酸(ΣSFA)含量下降, 吉富罗非鱼肌肉和肝脏ΣSFA 含量均呈下降的趋势; 随饲料亚油酸水平增加, 肌肉和肝脏的n-6 多不饱和脂肪酸(Σn-6 PUFA)含量呈上升趋势, 肌肉和肝脏的Σn-3 PUFA 含量呈下降趋势。综上所述, 初始体重为(60.98±3.82) g 的吉富罗非鱼饲料亚油酸需要量为1.02%~2.66%。

    Abstract:

    The Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus) strain is one of the more successfullyintroduced farmed tilapia in China because of its strong adaptability, rapid growth, high fecundity, andability to accept a broad diet. Similar to other fish and vertebrates, tilapia cannot synthesize 18-carbon polyunsaturatedfatty acids (PUFAs) and, thus, require a dietary source of n-6 series FAs (18: 2n-6 or 20: 4n-6) for normalgrowth and reproduction. Although the optimum n-6 FA dietary requirements for tilapia < 10 g have been estimatedto be 0.5%–1.0%, few studies have investigated the linoleic acid (LA) requirement for larger juvenile tilapia.Our objective was to determine the optimal dietary LA requirement for larger juvenile GIFT. A total of 630fish (mean body weight, 60.98±3.82 g) were divided randomly into seven groups with three replicates of 30 fish ineach replicate. Seven diets with a constant dietary lipid level (8%) were formulated to contain seven levels [0.07%(control group), 0.36%, 0.61%, 1.03%, 2.00%, 3.00%, and 4.15%] of LA by supplementation with corn oil andpalmitic acid to modulate FA contents. The fish were fed three times daily (8:30, 12:30, and 16:30) to apparentsatiation for 10 weeks. At the end of the feeding trial, growth performance, body composition, serum biochemicalindices, and FA composition were measured. The results showed that weight gain rate (WGR), feed efficiency rate(FER), protein efficiency rate, and protein retention rate (PRR) of GIFT increased initially and then decreased asLA level increased. A second-order regression analysis showed that the optimal LA level for the best WGR was2.49%, and that the dietary LA level for the best FER was 2.66%. Broken-line regression analyses of PRR againstdietary LA level indicated that the dietary LA level for the best PRR was 1.02%. Increasing dietary LA level contributedto increase tissue and whole-body lipid levels. Serum total cholesterol and triglyceride (TG) levels werelowest in the group fed 1.03% LA, whereas the 1.03% LA group had the highest high-density lipoprotein cholesterollevel. Low-density lipoprotein cholesterol content declined as LA increased. Broken-line regression analysesshowed that the optimum LA requirement for the minimum TG level was 1.13%. Tissues FA composition was affectedby dietary FA composition. Muscle and liver saturated fatty acid levels declined as dietary level decreased;however, muscle and liver n-6 FA levels increased and n-3 FA levels declined with the increase in LA level. Ourcomprehensive analysis of growth performance, serum biochemical indices, and FA compositio

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田娟,涂玮,文华,蒋明,吴凡,刘伟,杨长庚,黄凤.吉富罗非鱼对饲料亚油酸的需要量[J].中国水产科学,2016,23(1):104-116
TIAN Juan, TU Wei, WEN Hua, JIANG Ming, WU Fan, LIU Wei, YANG Changgeng, HUANG Feng. Optimal dietary linoleic acid requirement for the advanced juvenile GIFT strain of Nile tilapia, Oreochromis niloticus [J]. Journal of Fishery Sciences of China,2016,23(1):104-116

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