蚕豆、大巢菜籽及苦瓜籽饲料对草鱼生长、肌肉成分、组织生化指标和消化酶活性的影响
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作者单位:

1. 上海海洋大学水产与生命学院, 上海 201306;
2. 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306;
3. 上海市水产养殖工程技术研究中心, 上海 201306;
4. 上海高校知识服务平台, 上海海洋大学水产动物遗传育种中心(ZF1206), 上海 201306

作者简介:

郑小淼(1989-),男,硕士,研究方向为水产动物营养与饲料.E-mail:xmzheng19890417@163.com

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中图分类号:

S96

基金项目:

上海市自然科学基金项目(13ZR1419600).


Effects of broad bean, common vetch seed and bitter melon seed diets on the growth, flesh composition, tissue indices and digestive enzymes of grass carp
Author:
Affiliation:

1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
2. Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Aquaculture;Shanghai Ocean University, Shanghai 201306, China;
3. Shanghai Engineer

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    摘要:

    分别以常规配合饲料(配合饲料组)、浸泡蚕豆(浸泡蚕豆组)、含75%蚕豆(蚕豆饲料组)、60%大巢菜籽(巢菜籽饲料组)、60%苦瓜籽(苦瓜籽饲料组)的饲料饲喂体重为(59.0±0.6) g的草鱼(<0.05),饲料系数显著低于其他各组(P<0.05);浸泡蚕豆组、蚕豆饲料组、巢菜籽饲料组、苦瓜籽饲料组的肌肉胶原蛋白含量较配合饲料组显著提高,而肌肉脂肪含量显著下降(<0.05)。在生化指标方面,与配合饲料组相比,投喂蚕豆、巢菜籽饲料及浸泡蚕豆显著降低了肝胰脏超氧化物歧化酶(SOD)活性(<0.05),但蚕豆饲料组的肝胰脏丙二醛(MDA)含量低于浸泡蚕豆组(<0.05),提高了α-淀粉酶活性(<0.05)。上述结果表明,饲喂以蚕豆、大巢菜籽、苦瓜籽为主要成分的配合饲料及浸泡蚕豆可改变草鱼肌肉组成,但也降低了草鱼生长性能;综合生长性能和肌肉组成,蚕豆饲料较巢菜籽饲料、苦瓜籽饲料和浸泡蚕豆在脆化草鱼生产养殖上更具有可行性。

    Abstract:

    Five diets, including complete formula diet (control), soaked broad bean (SBB, fed as crumbled pieces after immersionin water), broad bean, common vetch seed and bitter melon seed diets (BBD, CVD, BMD), which included 75% broad bean, 60% common vetch seed and 60% bitter melon seed in formula diet, respectively, were fed to juvenile grass carps with an initial body weight of (59.0±0.6) g. After 8 weeks of feeding, the control fish had the highest weight gain (WG) and lowest feed conversation ratio (FCR) among all groups (<0.05). Compared with the SBB group, the WG was 56.9% in the BBD group, and -30.6% and -21.2% in the CVD and BMD groups (<0.05), respectively. Muscle collagen contents of the SBB, BBD, CVD and BMD groups were significantly higher, while muscle lipid contents were significantly lower than those of the control (<0.05). The BMD group had higher total amino acids and delicious amino acids contents in muscle than those of the control and SBB groups (<0.05). The hepatopancreatic superoxide dismutase (SOD) activities of fish fed the BBD, CVD and SBB diets were significantly lower (<0.05), and hepatopancreatic malonaldehyde (MDA) content and muscle creatine kinase (CK) activity were higher than those of fish fed the control diet (<0.05). However, fish fed the BBD diet had lower hepatopancreatic MDA than fish fed the SBB diet. Fish fed the BBD, CVD, BMD and SBB diets had a lower lipidase activities and a higher amylase activities in their intestines compared with the control fish (<0.05). The fish fed the BBD, CVD and SBB diets also showed lower protease activities than the control (<0.05). In conclusion, soaked broad bean and high levels of dietary broad bean, common vetch seeds and bitter melon seed saltered the muscle composition of grass carp, and significantly decreased their growth performance. For growth and muscle composition, a broad bean diet has more advantages in the culture of crisped grass carp than common vetch seed, bitter melon seed and soaked broad bean diets.

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郑小淼,李小勤,魏静,胡静,孙文通,徐怀兵,冷向军.蚕豆、大巢菜籽及苦瓜籽饲料对草鱼生长、肌肉成分、组织生化指标和消化酶活性的影响[J].中国水产科学,2016,23(2):370-381
ZHENG Xiaomiao, LI Xiaoqin, WEI Jing, HU Jing, SUN Wentong, XU Huaibing, LENG Xiangjun. Effects of broad bean, common vetch seed and bitter melon seed diets on the growth, flesh composition, tissue indices and digestive enzymes of grass carp[J]. Journal of Fishery Sciences of China,2016,23(2):370-381

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  • 在线发布日期: 2016-03-14
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