翘嘴鳜选育群体的生长和遗传特征分析
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作者单位:

1. 华南农业大学动物科学学院, 广东广州 510642;
2. 中山大学生命科学学院, 水生经济动物研究所, 南海生物资源开发与利用协同创新中心, 广东广州 510275;
3. 佛山市南海百容水产良种有限公司, 广东省工程技术研究中心, 广东佛山 528216

作者简介:

孙际佳(1976-),女,博士,主要从事水产动物遗传育种研究.E-mail:jjsun@scau.edu.cn

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中图分类号:

S96

基金项目:

广东省战略性新兴产业核心技术攻关项目(2012A020800001);广东省教育厅项目(cxzd1104);佛山市引进科技创新团队资助计划(2014IT100122).


Analysis of growth and genetic characteristics of the breeding populations of Siniperca chuatsi
Author:
Affiliation:

1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
2. South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center;Institute of Aquatic Economic Animals, School of Life Sciences, Sun

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    摘要:

    以湖南和江苏的野生翘嘴鳜(两个群体选育群体。对4个家系选育群体的生长特性进行了分析,并利用微卫星标记技术对6个翘嘴鳜选育群体的遗传多样性进行了检测。分析结果表明,翘嘴鳜纯种家系的生长速度显著快于不同地理种群的杂交系,杂交系未表现出生长的杂种优势,家系群体的杂合性与生长性能不对应。4个家系选育群体的特有等位基因数量比两个群体选育群体高出约29.33%,表明不同地理种群翘嘴鳜家系的建立可以丰富翘嘴鳜选育群体的遗传多样性水平。翘嘴鳜选育群体间遗传分化显著(=0.4388),两个群体选育群体与4个家系选育群体间的遗传距离较远,表明将群体选育的个体与家系选育的个体进行杂交育种有可能获得杂种优势。筛选到G14437特有等位基因可作为江苏翘嘴鳜家系(JCJC)的群体特异性分子标记; G5530等位基因可作为湖南和江苏翘嘴鳜杂交系与翘嘴鳜基础群体和其他选育群体区分的特异性分子标记。

    Abstract:

    Based on the wild base-populations sampled from Hunan and Jiangsu provinces, six breeding populations of , including four pedigree-bred and two population-bred populations, were cultured. The growth properties of four pedigree-bred populations were studied and the genetic characteristics of all six breeding populations were analyzed with seven microsatellite DNA markers. The growth speed of the pure-bred families were significantly higher than geographic hybrid families, but there was no direct relationship between the growth rates and heterozygosities. The number of private alleles of the pedigree-bred populations was about 29.33% more than that of the two population-bred populations. This means that the establishment of family with different geographic populations might raise the level of genetic diversity. The genetic differentiation within breeding populations was significant (=0.4388). The relative farther genetic distance between the pure-bred populations and the pedigree-bred populations revealed that the hybridization between them might obtain some offsprings which exhibited heterosis. Private allele G14437 could be used as the specific molecular markers for JCJC population, while G5530 used to distinguish the geographic hybrid populations (HCJC and JCHC) from other breeding populations.

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引用本文

孙际佳,何建国,刘丽,王海芳,卢薛,胥鹏,李桂峰.翘嘴鳜选育群体的生长和遗传特征分析[J].中国水产科学,2016,23(2):425-435
SUN Jijia, HE Jianguo, LIU Li, WANG Haifang, LU Xue, XU Peng, LI Guifeng. Analysis of growth and genetic characteristics of the breeding populations of Siniperca chuatsi[J]. Journal of Fishery Sciences of China,2016,23(2):425-435

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  • 在线发布日期: 2016-03-14
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