The white shrimp, (Decapoda, Penaeidae), isomnivorous, grows quickly, and has a low food nutrition demand, which has made it an economically important crustacean species in China. However, the single breed type and high-density culture can result in bacterial and viral disease outbreaks. is the dominant bacteria in shrimp and causes disease when shrimp are immunocompromised. In this study, (initial body length, 7.90 cm±1.15 cm; initial body weight, 7.20 g±1.38 g) were purchased from Qingdao Baorong Aquaculture Co., Ltd. A 3-week feeding experiment and a 2-week infection experiment were performed to evaluate the effects of adding the probiotic to feed on promoting intestinal bacteria, non-specific immune gene expression, and resistance to . The experimental shrimp were divided into control and experimental groups. Control group shrimp were fed commercial feed throughout the experiment, and the experimental group shrimp were fed the same feed supplemented with 1.0×10⁸ CFU/mL and total intestinal bacteria in were checked every 7 days using 22l6E and TCBS media. After 3 weeks of feeding, all shrimp were injected with 1.0×10⁷ CFU/mL (50 μL; dose determined in preliminary experiment).Then, 6 h, 12 h, 18 h, 24 h, 36 h, 48 h, and 72 h, as well as 7 d later, three shrimp from each group were captured randomly to extract RNA from the gill. The RNA was reversed transcribed into cDNA and tested for lysozyme and Toll receptor expression levels. The cumulative mortality rate of the control group was 100%, and the cumulative mortality rate of the experimental group was 22.78% after 14 days. The relative protection ratio was 22.22%. The experimental feed significantly reduced the number of P < 0.05). Relative lysozyme mRNA expression levels in the experimental group 12 h, 18 h, 24 h, 36 h, 48 h, and 72 h after the challenge were significantly higher than thosein the control group( < 0.05). Relative Toll receptor mRNA expression levels in the experimental group 6 h, 12 h, 18 h, 24 h, 36 h, 48 h, and 72 h, as well as 7 d after the challenge were significantly higher than those in the control group (to shrimp feed effectively improved the ability of infection, possibly by reducing the number of in the intestine and improving immune-related gene expression.