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三疣梭子蟹卵巢发育过程中雌二醇的免疫定位和变化
作者:
作者单位:

1. 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306;
2. 上海海洋大学 上海市水产动物遗传育种协同创新中心, 上海 201306

作者简介:

柳梅梅(1990),女,硕士研究生,主要从事甲壳动物繁殖生物学的研究.E-mail:15105517287@163.com

中图分类号:

S96

基金项目:

国家自然科学基金项目（41276158）；上海市自然科学基金项目（12ZR1413000）；上海市科技兴农重点攻关项目[（沪农科攻字（2013）第6-3号）]；上海市科技兴农推广项目[沪农科攻字（2016）第1-1-8号]；上海市高峰学科建设项目（2012-62-0908）；浙江省东海海水养殖产业升级协同创新中心项目.


Immunolocalization and changes in 17β-estradiol in Portunus trituberculatus during ovarian development
Author:
Affiliation:

1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education;Shanghai Ocean University, Shanghai 201306, China;
2. Collaborative Innovation Center of Aquatic Animal Breeding Center Certified by the Shanghai Municipal Education Commission;Shanghai Ocean University, Shanghai 201306, China

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    摘要:

    雌二醇（estradiol，E2）是甲壳动物体内重要的性类固醇激素，对其卵巢发育和卵黄发生起着十分重要的调控作用。本实验采用免疫组织化学方法系统研究了三疣梭子蟹（在卵巢、肝胰腺、胸神经节、脑神经节和大颚器中的分布及变化。结果表明：（1）蟹卵巢中E2的免疫阳性主要分布于滤泡细胞和卵巢发育中后期的卵母细胞细胞质（II-V期），卵巢发育过程中卵母细胞细胞质和细胞核中的E2免疫阳性均为先上升后下降的趋势，最大免疫阳性分别出现在IV期和II-III期，滤泡细胞中始终存在较强的E2免疫阳性；（2）肝胰腺中的E2免疫阳性主要分布于F细胞及R细胞细胞核中，R细胞细胞核中的E2免疫阳性从III期开始显著下降，F细胞中始终存在较强的E2免疫阳性；（3）卵巢发育（I-V期）的神经组织，E2主要分布于胸神经团的神经细胞和神经髓质以及脑神经节神经细胞细胞核内，其中脑神经节神经细胞细胞核中始终保持着强免疫阳性，卵巢发育早期（I-II期）胸神经团中的神经髓质中为E2中等免疫阳性，神经细胞中一直为弱免疫阳性；（4）就大颚器而言，E2强免疫阳性始终存在于大颚器腺细胞的核仁以及细胞核周围的细胞质内。以上结果表明，E2在三疣梭子蟹卵巢、肝胰腺、神经组织和大颚器中广泛分布，且免疫阳性与卵巢发育具有一定的相关性，E2可能通过作用于多个靶器官来调控三疣梭子蟹的卵巢发育。

    Abstract:

    17β-Estradiol (E2) is among the most important sex steroid hormones during ovarian development and vitellogenesis in crustaceans. In this study, an immunofluorescence assay was used to identify the immunopositive distribution and variations in E2 in the ovary, hepatopancreas, thoracic ganglia, brain ganglion, and the mandibular organ during ovarian development in the swimming crab, was mainly distributed in the follicular cells and the cytoplasm of late-stage oocytes (II-V). Follicular cells stained strongly positive for E2 at all ovarian stages; the immunoreactivities of E2 in the oocyte cytoplasm and nucleus trended as "low-high-low", and maximum positive staining appeared during ovarian stage IV and stages II-III, respectively. In the hepatopancreas, E2-positive staining was mainly distributed in F cells and R nuclei, and E2 immunoreactivity in the R nucleus tended to decrease from stages III to IV. However, strong positive E2 immunoreactivity was always detected in F cells at all ovarian stages. E2 was mainly distributed in neurons, the nerve medulla of thoracic ganglia, and cell nuclei of cerebral ganglion nerve cells during the early stages of ovarian development (I-II). E2 immunore activity in the nerve medulla of the thoracic ganglion was moderately positive, where as neurons were always weakly positive. Strong positive E2 staining was always distributed in the nuclei of mandibular gland cells and the cytoplasm around mandibular gland cellnuclei. These results suggest that E2 is widely distributed in the ovary, hepatopancreas, thoracic ganglion, cerebral ganglion, and mandibular gland of immunoreactivity and ovarian developmental stage. E2 may act through multiple target organs to regulate ovarian development in

    参考文献
    [1] Lu J F, Zhao W X. Effect and regulation of reproductive hormones on ovary in decapod crustacean[J]. Journal of Shanghai Fisheries University, 2001, 10(2):166-171.[陆剑锋, 赵维信. 十足目甲壳动物生殖激素对卵巢的作用及其调控[J]. 上海水产大学学报, 2001, 10(2):166-171.]
    [2] Quinitio E T, Hara A, Yamauchi K, et al. Changes in the steroid hormone and vitellogenin levels during the gametogenic cycle of the giant tiger shrimp, Penaeus monodon[J]. Comp Biochem Physiol C:Pharmacol Toxicol Endocrinol, 1994, 109(1):21-26.
    [3] Huang H Y, Ye H H, Han S Z, et al. Profiles of gonadotropins and steroid hormone-like substances in the hemolymph of mud crab Scylla paramamosain during the reproduction cycle[J]. Mar Freshw Behav Physiol, 2009, 42(4):297-305.
    [4] Ye H H, Ai C X, Huang H Y, et al. Advances on reproductive physiology of crabs[J]. Journal of Xiamen University:Natural Science, 2006, 45(z2):170-175.[叶海辉, 艾春香, 黄辉洋, 等. 蟹类生殖生理学研究进展[J]. 厦门大学学报:自然科学版, 2006, 45(z2):170-175.]
    [5] Cai S L, Zhao W X, Yang C H. Detection of progesterone and estradiol, and observation of histology in the mandibular organ of shrimp Fenneropenaeus chinensis[J]. Acta Hydrobiologica Sinica, 2007, 31(2):290-296.[蔡生力, 赵维信, 杨丛海. 中国明对虾大颚器的组织学及孕酮和雌二醇的检测[J]. 水生生物学报, 2007, 31(2):290-296.]
    [6] Martins J, Coimbra J. Reproductive cycle, ovarian development, and vertebrate-type steroids profile in the freshwater prawn Macrobrachium rosenbergii[J]. J Crustac Biol, 2009, 27(2):220-228.
    [7] Feng L. The effect of dietary HUFA on the ovary development, endocrine hormones and tissue biochemical composition of the swimming crab Portunus trituberculatus[D]. Shanghai:Shanghai Ocean University, 2011:1-81.[丰浪. 高不饷和脂肪酸(HUFA)对三疣梭子蟹卵巢发育、内分泌激素以及组织生化组成的影响[D]. 上海:上海海洋大学, 2011:1-81.]
    [8] Zhang Y. Developmental changes in concentrations of vitellin, vitellogenin and vitellogenin gene expression during the ovarian development of swimming crab, Portunus trituberculatus[D]. Shanghai:Shanghai Ocean University, 2011:1-76.[张艳. 三疣梭子蟹卵巢发育期间卵黄蛋白原(Vg)、卵黄磷蛋白(Vn)及Vg-mRNA表达的变化研究[D]. 上海:上海海洋大学, 2013:1-76.]
    [9] Yano I, Hoshino R. Effects of 17β-estradiol on the vitellogenin synthesis and oocyte development in the ovary of kuruma prawn (Marsupenaeus japonicus)[J]. Compar Biochem Physiol A:Mol Integr Physiol, 2006, 144(1):18-23.
    [10] Shen B J, Yang X Z, Wu X G, et al. The effects of exogenous 17β-estradiol on ovary development and on the level of endogenous 17β-estradiol in Eriocheir sinensis[J]. Journal of Shanghai Ocean University, 2010, 19(3):289-295.[沈蓓杰, 杨筱珍, 吴旭干, 等. 外源17β-E2对中华绒螯蟹Ⅲ-Ⅳ期卵巢发育以及内源雌激素水平的影响[J]. 上海海洋大学学报, 2010, 19(3):289-295.]
    [11] Liu Z J. A study on estrogen and its estrogen related receptor during the ovarian development of swimming crab, Portunus trituberculatus[D]. Shanghai:Shanghai Ocean University, 2013:1-73.[刘智俊. 三疣梭子蟹卵巢发育期间雌激素受体分布变化及雌激素离体调控的研究[D]. 上海:上海海洋大学, 2013:1-73.]
    [12] Wu X G, Cheng Y X, Zeng C S, et al. Reproductive performance and offspring quality of wild-caught and pond-reared swimming crab Portunus trituberculatus broodstock[J]. Aquaculture, 2010, 301:78-84.
    [13] Wu X G, Cheng Y X, Zeng C S, et al. Reproductive performance and offspring quality of the first and the second brood of female swimming crab, Portunus trituberculatus broodstock[J]. Aquaculture, 2010, 303:94-100.
    [14] Newton S S, Dow A, Terwilliger R, et al. A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections[J]. Rev Árvore, 2002, 38(3):214-219.
    [15] Osada M, Tawarayama H, Mori K. Estrogen synthesis in relation to gonadal development of Japanese scallop, Patinopecten yessoensis:gonadal profile and immunolocalization of P450 aromatase and estrogen[J]. Comp Biochem Physiol B:Biochem Mol Biol, 2004, 139(1):123-128.
    [16] Wu X G, Yao G G, Yang X Z, et al. A study on the ovarian development of Portunus trituberculatus in East China Sea during the first reproductive cycle[J]. Acta Oceanologica Sinica, 2007, 29(4):120-128.[吴旭干, 姚桂桂, 杨筱珍, 等. 东海三疣梭子蟹第一次卵巢发育规律的研究[J]. 海洋学报, 2007, 29(4):120-128.]
    [17] Yao G G, Wu X G, Cheng Y X, et al. The changes of histology and main biochemical composition in the hepatopancreas at the different physiological stages of Portunus trituberculatus in East China Sea[J]. Acta Oceanologica Sinica, 2008, 30(6):122-131.[姚桂桂, 吴旭干, 成永旭, 等. 东海三疣梭子蟹雌体不同生理阶段肝胰腺的生化组成与其组织学结构的关系[J]. 海洋学报, 2008, 30(6):122-131.]
    [18] Couch E F, Hagino N, Lee J W. Changes in estradiol and progesterone immunoreactivity in tissues of the lobster, Homarus americanus, with developing and immature ovaries[J]. Comp Biochem Physiol A:Physiol, 1987, 87(3):765-770.
    [19] Summavielle T, Monteiro P R R, Reis-Henriques M A, et al. In vitro metabolism of steroid hormones by ovary and hepatopancreas of the crustacean Penaeid shrimp Marsupenaeus japonicus[J]. Sci Mar, 2003, 67(3):299-306.
    [20] Jia X, Chen Y, Zou Z, et al. Characterization and expression profile of vitellogenin gene from Scylla paramamosain[J]. Gene, 2013, 520(2):119-130
    [21] Cheng Y X, Li S J, Wang G Z, et al. Structural modulation of the area between oocytes and follicular cells during vitellogenesis of the mud crab(Scylla serrata)[J]. Acta Zoologica Sinica, 2002, 48(1):80-92.[成永旭, 李少菁, 王桂忠, 等. 锯缘青蟹卵黄发生期卵母细胞和卵泡细胞之间的结构变化[J]. 动物学报, 2002, 48(1):80-92.]
    [22] Lu J F, Chang G L, Wu X G, et al. Hormonal regulations of ovarian development and vitellogenesis in Chinese mitten crab Eriocheir sinensis feed on two different diets[J]. Oceanologia et Limnologia Sinica, 2010, 41(4):505-512.[陆剑锋, 常国亮, 吴旭干, 等. 两组不同饲料对中华绒螯蟹(Eriocheir sinensis)卵巢发育及卵黄发生的激素调控[J]. 海洋与湖沼, 2010, 41(4):505-512.]
    [23] Lee F Y, Chang C F. The concentrations of vitellogenin (Vitellin) and protein in hemolymph, ovary and hepatopancreas in different ovarian stages of the Freshwater Prawn, Macrobrachium rosenbergii[J]. Comp Biochem Physiol A:Physiol, 1997, 117(4):433-439.
    [24] Soroka Y, Milner Y, Sagi A. The hepatopancreas as a site of yolk protein synthesis in the prawn Macrobrachium rosenbergii[J]. Invert Reprod Dev, 2000, 37(1):61-68.
    [25] Mazurová E, Hilscherová K, Triebskorn R, et al. Endocrine regulation of the reproduction in crustaceans:identification of potential targets for toxicants and environmental contaminants[J]. Biologia, 2008, 63(2):139-150.
    [26] Raviv S, Parnes S, Sagi A. Coordination of reproduction and molt in decapods[M]//Mente E. Reproductive Biology of Crustaceans:Case Studies of Decapod Crustaceans. Science Publishers, 2008:365-390.
    [27] Nagaraju G P C. Reproductive regulators in decapod crustaceans:an overview[J]. J Exp Biol, 2011, 214(1):3-16.
    [28] Ye H H, Huang H Y, Li S J, et al. Immunological recognition of FSH and LH in the brain ganglion of the mud crab, Scylla serrata[J]. Progress in Natural Science, 2006, 16(6):768-770.[叶海辉, 黄辉洋, 李少菁, 等. 锯缘青蟹(Scylla serrata)脑中FSH和LH的免疫识别[J]. 自然科学进展, 2006, 16(6):768-770.]
    [29] Kalló I, Butler J A, Barkovics-Kalló M, et al. Oestrogen receptor beta-immunoreactivity in gonadotropin releasing hormone-expressing neurones:regulation by oestrogen[J]. J Neuroendocrinol, 2001, 13(9):741-748.
    [30] Nagaraju G P C, Reddy P R, Reddy P S. Mandibular organ:its relation to body weight sex molt and reproduction in the crab Oziotelphusa senex senex Fabricius (1791)[J]. Aquaculture, 2004, 232:603-612.
    [31] Nagaraju G P C. Is methyl farnesoate a crustacean hormone?[J]. Aquaculture, 2007, 272:39-54.
    [32] Li S, Zhao W X. Mandibular organ and methyl farnesoate in crustacean[J]. Journal of Shanghai Fisheries University, 2000, 9(3):240-244.[李胜, 赵维信. 甲壳动物的大颚器和甲基法尼酯[J]. 上海水产大学学报, 2000, 9(3):240-244.]
    [33] Takáč P, Ahl J S B, Laufer H. Methyl farnesoate binding proteins in tissues of the spider crab, Libinia emarginata[J]. Comp Biochem Physiol B:Comp Biochem, 1998, 120(4):769-775.
    [34] Lu J F, Bai H, Cheng Y X, et al. In vitro regulation of hormone biosynthesis of mandibular organ by progesterone in crayfish, Procambarus clarkia[J]. Journal of Fishery Sciences of China, 2006, 13(3):476-479.[陆剑锋, 白桦, 成永旭, 等. 孕酮对克氏原螯虾大颚器激素生物合成的体外调控[J]. 中国水产科学, 2006, 13(3):476-479.]
    [35] Köhler H R, Kloas W, Schirling M, et al. Sex steroid receptor evolution and signalling in aquatic invertebrates[J]. Ecotoxicology, 2007, 16(1):131-143.
    [36] Kim K H, Moriarty K, Bender J R. Vascular cell signaling by membrane estrogen receptors[J]. Steroids, 2008, 70:382-387.
    [37] Thomas P, Tubbs C, Berg H, et al. The Fish Oocyte[M]. Amsterdam:Springer, 2007:203-233.
    [38] Liu X, Zhu P, Sham K W, et al. Identification of a membrane estrogen receptor in zebrafish with homology to mammalian GPER and its high expression in early germ cells of the testis[J]. Biol Reprod, 2009, 80(6):1253-1261.
    [39] Colbourne J K, Pfrender M E, Gilbert D, et al. The ecoresponsive genome of Daphnia pulex[J]. Science, 2011, 331(6017):555-561.
    [40] Thornton J W, Need E, Crews D. Resurrecting the ancestral steroid receptor:Ancient origin of estrogen signaling[J]. Science, 2003, 301(5640):1714-1717.
    [41] Giguère V. To ERR in the estrogen pathway[J]. Trends Endocrinol Metabol Tem, 2002, 13(5):220-225.
    [42] Cheung C P, Yu S, Wong K B, et al. Expression and functional study of estrogen receptor-related receptors in human prostatic cells and tissues[J]. J Clin Endocrinol Metabol, 2005, 90(3):1830-1844.
    [43] Tremblay A M, Giguère V. The NR3B subgroup:an ovERRview[J]. Nucl Recept Signal, 2007, 5:e009.
    [44] Tennessen J M, Baker K D, Lam G, et al. The Drosophila estrogen-related receptor directs a metabolic switch that supports developmental growth[J]. Cell Metabol, 2011, 13(2):139-148.
    [45] Danielsen M, Hinck L, Ringold G. Two amino acids within the knuckle of the first zinc finger specify DNA response element activation by the glucocorticoid receptor[J]. Cell, 1989, 57(7):1131-1138.
    [46] Deblois G, Giguère V. Functional and physiological genomics of estrogen-related receptors (ERRs) in health and disease[J]. Biochimica et Biophysica Acta, 2011, 1812(8):1032-1040.
    [47] Liu D, Zhang Z, Gladwell W, et al. Estrogen stimulates estrogen-related receptor alpha gene expression through conserved hormone response elements[J]. Endocrinology, 2003, 144(11):4894-4904.
    [48] Tarrant A M, Greytak S R, Callard G V, et al. Estrogen receptor-related receptors in the killifish Fundulus heteroclitus:diversity, expression, and estrogen responsiveness[J]. J Mol Endocrinol, 2006, 37(1):105-120.
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柳梅梅,吴旭干,潘杰,成永旭.三疣梭子蟹卵巢发育过程中雌二醇的免疫定位和变化[J].中国水产科学,2017,24(2):239-247
LIU Meimei, WU Xugan, PAN Jie, CHENG Yongxu. Immunolocalization and changes in 17β-estradiol in Portunus trituberculatus during ovarian development[J]. Journal of Fishery Sciences of China,2017,24(2):239-247

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