A 56-d feeding experiment was conducted to investigate the effects of replacing fish meal in the diet with soy protein concentrate (SPC) on growth, body composition, serum biochemical indices, and liver histology of juvenile large yellow croaker, (initial weight 10.50 g±0.04 g). The basal diet contained 40% fish meal, and five isonitrogenous (crude protein 45%) and isolipidic (crude lipid 10%) diets were formulated by replacing 0%, 25%, 50%,75%, and 100% of the fish meal with SPC and were designated the FM, R25, R50, R75, and R100 groups, respectively. All diets were supplemented with the appropriate amounts of crystalline lysine and methionine, based on lysine and methionine contents in the control group. Each diet was assigned randomly to triplicate groups of 60 ind/cage. All larvae were stocked into experimental cages (1.5 m×1.5 m×2 m) at Xihugang, Xiangshan County, Ningbo city, Zhejiang Province, China. As results, none of the experimental diets had a significant effect on the weight gain rate, specific growth rate, survival rate, or feed conversion ratio compared with those of fish fed the FM diet (>0.05). Whole-body, muscle, and crude lipid contents decreased as the SPC replacement level was increased, and fish fed the R100 diet had the lowest contents. However, whole-body and muscle moisture contents increased as the SPC replacement level was increased. No differences in any of the serum biochemical indices were observed among the groups (>0.05); briefly, serum total protein and albumin contents decreased, and fish fed the R100 diet had the lowest contents. Cholesterol and triglycerides tended to increase initially, and then decreased. Liver histology revealed serious damage to hepatocytes when SPC reached 75% (R75), as large cytoplasmic vacuole-like spaces were present, indicating excess fat accumulation, and nuclei had dissolved gradually or were absent in many of these cells. These results suggest that protein from SPC can substitute for up to 75% of the fish meal protein in the diet without affecting growth of .