Kinetic parameters were determined for the biological accumulation and release of lead (Pb) in muscle, the hepatopancreas, and exoskeleton of to assess the enrichment and release characteristics of Pb in seawater, feed, and tissues using a two-compartment bioconcentration model. Shrimp were exposed to seawater with Pb concentrations of 0.0015 mg/L(B0), 0.0080 mg/L(B1), 0.0466 mg/L(B2), and 0.2302 mg/L(B3). Shrimp were provided a diet with Pb concentrations of 2.089 mg/kg(A0), 2.750 mg/kg(A1), 6.103 mg/kg(A2), and 14.520 mg/kg(A3). The heavy metal uptake rate constant (), bioconcentration factor (BCF), biological half-life(in vivo () were obtained by non-linear curve fitting. The results showed that muscle, exoskeletal, and hepatopancreatic tissue contents were less <0.5 mg/kg after feeding Pb. Pb in all tissues increased to >0.5 mg/kg after exposing shrimp to the two lower Pb concentrations in seawater as the Pb concentration in feed was increased in shrimp in the B3 seawater treatment. The Pb release rate by the hepatopancreas was higher than that by muscle or the exoskeleton. A three factor repeated-measures analysis of variance with feed concentration, seawater concentration, and enrichment time as the factors showed that all three factors had significant effects in the different tissues. In addition, the Pb accumulating effect in the exoskeleton based on feed concentration was marginally significant (=0.071). A feed, seawater, and time interaction effect analysis resulted in a significant interaction among the three factors. An analysis of Pb feed and seawater concentrations, as well as Pb duration in different tissues showed that the contribution of Pb concentration in seawater was greater than that in feed during Pb enrichment in each tissue. At steady state, the Pb concentrations in muscle of shrimp in the A0 and B0-B3 groups were 0.128-2.981 mg/kg, and those in the hepatopancreas were 0.399-4.765 mg/kg. The values for Pb in the muscle and hepatopancreas were 2-7 d and 3-7 d, respectively. The Pb concentrations in muscle of shrimp from the A2 and B0-B3 groups were 0.380-1.000 mg/kg, and in the hepatopancreas were 0.288-5.355 mg/kg. The values for Pb in muscle and hepatopancreas were 2-7 d and 2-5 d. The steady state concentration of Pb in the hepatopancreas was higher than that in muscle.