This study determined the effects of different sources of dietary lipid on survival rate, weight gain, molting, digestive enzyme activity, and fatty acid composition in male juvenile Chinese mitten crab, . Five diets were formulated to contain different amounts of fish oil, soybean oil, and linseed oil. The control feed (Group F1) was formulated with 6% fish oil, whereas the other 4 experimental diets were formulated with 6% soybean oil (Group F2), 6% linseed oil (Group F3), 3% fish oil and 3% soybean oil (Group F4), and 3% fish oil and 3% linseed oil (Group F5). Juvenile crabs (with initial body weight 2.15 g±0.10 g) were randomly allocated to one of the diet groups. Twelve juvenile crabs per diet group were individually stocked in plastic tanks. After 112 d, weight gain and specific growth rate of Group F5 was significantly higher than that of the other groups (<0.05). No significant difference was observed in molting interval or hepatosomatic index. Analysis of digestive enzyme profiles from the hepatopancreas revealed positive correlations between enzyme activity and lipid sources:tryptase activity in Group F1 was significantly higher than that in the other groups (<0.05); pepsin activity in Group F3 was significantly higher than that in the other groups (<0.05); lipase activity in Groups F1, F2, and F4 was significantly higher than that in the other two groups (<0.05). Different diets had no significant effects on amylase activity. Fatty acid composition of the hepatopancreas and muscles followed the fatty acid composition of the diet. Eicosapentaenoic acid (C_20:5n3) and docosahexaenoic acid (C_22:6n3) in Group F1 was significantly higher than that in the other groups () in Group F2 was significantly higher than that in the other groups () in Group F3 was significantly higher than that in the other groups (<0.05). These results suggested that replacing 50% fish oil with soybean or linseed oil had a positive effect on growth, but a negative effect on survival rate in juvenile Chinese mitten crab, . Both soybean oil and linseed oil had a significant effect on tryptase activity, pepsin activity, lipase activity, and fatty acid of the hepatopancreas and muscles in juvenile Chinese mitten crab, .