Azaspiracids (AZAs) are a group of lipophilic polyether compounds first discovered in Ireland and have been implicated in shellfish poisoning incidents. Known toxin-producing genera have rapidly increased and now comprise six species, including A. dexteroporum. Approximately 40 different analogs have been reported to differ in structure and toxicity, of which AZA1, AZA2, and AZA3 are the most important ones based on occurrence and toxicity. AZAs are a variety of nitrogen-containing polyether toxins, which include a unique spiral ring assembly, a heterocyclic amine, and an aliphatic carboxylic acid moiety. The consumption of polluted shellfish can cause human intoxication, with symptoms including nausea, vomiting, diarrhea and stomach cramps, which is similar to diarrhetic shellfish poisoning (DSP). Nowadays, in order to guarantee food security, the developed countries have begun to prioritize shellfish monitoring. A regulatory level of 160 μg AZA1eq/kg in the shellfish tissues is implemented in many countries all over the world, but our country does not have a regulation about this. Amphidomataceae, which is known for the production of AZAs. Twenty two out of 25 strains isolated in China have proven to be AZA-producing strains, and show a large variability in the AZA profile among different strains. This status underlines the high-risk potential of blooms with subsequent shellfish intoxication for the Asian Pacific Ocean. AZA accumulation by bivalve mollusks occurs frequently, while blue mussels were found to accumulate by far the highest concentration. Meanwhile, AZAs have been found in various species of bivalve mollusks such as oysters, mussels, scallops, and clams along Chinese coastline. As AZA is a recently discovered toxin, the bloom dynamics, transfer kinetics, and pathways of AZAs into bivalve mollusks are just beginning to be explored. Accumulation and distribution of AZA toxins in bivalve shellfish could be correlated in time and space to blooms of species. Subsequent accumulation and trophic transfer can intoxicate higher trophic-level consumers and eventually pass into human beings. To investigate the hazard formation process, two transfer routes must be taken into consideration:AZAs could be accumulated by bivalve shellfish or plankton vectors from toxin producing algae. In conclusion, a direct link between AZA accumulation by bivalve shellfish and must be investigated for food safety control and monitoring plans. Liquid chromatography coupled with quadrupole linear ion trap tandem mass spectrometry was established for the evaluation of AZA accumulation, distribution, and biotransformation in scallops. Toxin-producing alga Chlamys farreri. The results showed that AZA2 is the dominant toxin of AZDY06, with toxin production capacity up to (7.05 ±0.52) fg/cell. After 12 h exposure experiments with 5x10⁷ cells AZDY06 ingested by every , AZAs in mussels reached the highest concentration and exceeded EU regulatory limits. The concentration was 165.3 μg AZA1eq/kg, while total accumulation efficiency reached 78.2%. The distribution of AZA toxins showed significant differences between organs, with gill > viscera > pallium > muscle. Four AZA analogs including AZA19, AZA12, AZA6, and AZA23 were found in all organs. AZA19 was the main metabolite with a proportion of 40%, while other metabolites were found more rarely. This study proved that the hazard of (AZDY06) isolated from China is strong and underlined that AZA19 was present at significant levels after only 6 h and until the end of the study. Therefore, this analog needs to be considered as the major metabolite that can affect human health. It is highly recommended to quickly formulate AZA limits, based on this analysis of raw bivalves by LC-MS/MS.