河南师范大学 水产学院, 河南省水产动物养殖工程技术研究中心, 河南 新乡 453007
刘慧芬(1985-),女,讲师,博士,研究方向为鱼类繁殖生理学和分子生态学.E-mail:huifen_liu@126.com
S931
河南省科技攻关重点项目（142102110057，162102310443，162102110147，172102310751）；河南省高校科技创新团队支持计划（14IRTSTHN013）；河南省创新型科技团队支持计划（CXTD2016043）.
College of Fisheries, Henan Normal University, Engineering Technology Reasearch Center of Henan Province for Aquatic Animal Cultivation, Xinxiang 453007, China
为准确掌握河南野生宽鳍鱲（）群体遗传结构，本研究利用线粒体CO I基因检测了海河、黄河、淮河和长江水系宽鳍鱲群体的遗传多样性和种群分化情况。110个样品共检测出40个单倍型，平均单倍型多样性为0.92077，平均核苷酸多样性为0.02439。AMOVA分析显示，大多数遗传变异存在于宽鳍鱲群体间（60.59%），群体内的遗传变异为37.67%。系统发育树结果表明，长江水系宽鳍鱲群体遗传分化较大，一部分群体单独聚为一支，另外一部分群体与海河、黄河、淮河水系宽鳍鱲聚在一起，没有显示出明显的南北分化格局。种群历史动态结果推测宽鳍鱲群体经历过瓶颈效应，且该过程可能与第四纪中更新世气候波动有关。建议对河南境内的宽鳍鱲种群，特别是遗传多样性低的种群（海河水系）进行保护。
To correctly determine the genetic structure of wild populations from Haihe, Yellow, Huaihe, and Yangtze River basins in Henan Province, we analyzed the population genetic diversity and differentiation of the species using mitochondrial CO I gene. A total of 110 individuals were sequenced, which have defined 40 distinct haplotypes. The average haplotype diversity and nucleotide diversity were 0.92077 and 0.02439, respectively. Analysis of molecular variance (AMOVA) indicated that most of the genetic variation existing among populations (60.59%), and the remaining 37.67% of genetic variation was attributed to differentiation within populations. The assignment of populations based on Neighbor-Joining (NJ) method revealed that populations from Yangtze River divided into two distinct mitochondrial DNA lineages, one clustered from the ZY population and the other clustered individuals from Haihe River, Yellow River and Huaihe River. This phylogenetic pattern is not consistent with the previous reported North and South Clades. Demographical history analysis indicated that populations had undergone a bottleneck which coincided with the middle Pleistocene and suffered from climate changes. Considering the genetic and phylogeographic results obtained in this study, appropriate measures are proposed to conserve population, especially for those with low genetic diversity in Haihe River basin.
刘慧芬,张超,王静,顾钱洪,周传江,孟晓林,张建新,宋东蓥,李学军,孔祥会,聂国兴.河南境内四水系宽鳍鱲野生群体的遗传多样性[J].中国水产科学,2018,25(2):269-277
LIU Huifen, ZHANG Chao, WANG Jing, GU Qianhong, ZHOU Chuanjiang, MENG Xiaolin, ZHANG Jianxin, SONG Dongying, LI Xuejun, KONG Xianghui, NIE Guoxing. Genetic diversity of wild Zacco platypus populations among four river systems in Henan Province analyzed by mitochondrial CO I gene[J]. Journal of Fishery Sciences of China,2018,25(2):269-277
