齐口裂腹鱼SNP标记与生长性状的关联分析
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西南大学 鱼类繁育与健康养殖研究中心, 重庆 402460

作者简介:

杨月静(1993-),女,硕士研究生,主要从事水产动物遗传育种与生物技术研究.E-mail:yangyuejing666@163.com

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S917

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国家自然科学基金资助项目(31402302);中央高校基本科研业务费资助项目(XDJK2017B008);西南大学荣昌校区青年基金资助项目(20700938).


Association analysis between SNP markers and growth-related traits in Schizothorax prenanti
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Fisheries Breeding and Health Cultivation Research Center, Southwest University, Chongqing 402460, China

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    摘要:

    齐口裂腹鱼()是中国特有的冷水性经济鱼类,具有较高的营养和经济价值。为了研究齐口裂腹鱼生长性状关联的分子标记,本研究以114尾同批次繁殖、相同养殖条件的齐口裂腹鱼为研究材料,运用26个齐口裂腹鱼SNPs位点进行生长性状(体重、体高、全长、体长)关联分析。对齐口裂腹鱼生长性状的主成分分析表明,体重占解释方差的93.42%,且特征根大于1,累积方差大于85%,是齐口裂腹鱼生长性状的第一主成分。SNPs位点与生长性状相关分析结果显示:对全长、体长有显著影响(ug22712-0-2452对体重、体高、全长和体长都有极显著影响(<0.01)。对与生长性状显著相关的4个SNPs位点进行多态性检测,平均观测杂合度和期望杂合度分别为0.2369和0.2110,平均多态信息含量为0.18,其中ug22712-0-2452与生长性状具有显著相关性,可作为候选位点用于齐口裂腹鱼的分子标记辅助育种。本研究旨为齐口裂腹鱼的遗传改良和选择育种提供基础资料。

    Abstract:

    , an economically important, cold-water fish species, has rich nutritional value and high economic value. With the increase in artificial breeding and breeding intensification, the germplasm resources of have been degraded, which is revealed in individual miniaturization, slow growth, and decreased disease resistance. Therefore, it is necessary to screen the molecular markers of growth traits and use molecular-marker-assisted breeding. In order to acquire some reliable molecular genetic markers for growth-related traits, the correlation analysis of 26 SNP markers and growth-related traits in were analyzed using 114 samples with the same growth conditions. A principal component analysis showed that body weight accounted for 93.42% of the variance, the eigenvalue was greater than 1 and the accumulative variance ratio was more than 85%, and it was the first principal component of the growth traits of . Correlation analysis between genotypes of SNPs and growth traits indicated that <0.05), and showed a significant influence on total length and body length (ug25050-0-1678 and body weight, body height, total length, and body length ( and the body weight, body height, and body length were significantly associated (<0.01). We also estimated that the genetic diversity parameters for 4 loci were significantly correlated with the growth traits. The mean observed heterozygosity, expected heterozygosity, and polymorphism information content (PIC) were 0.2369, 0.2110 and 0.17 respectively. The polymorphism of ug25050-0-1678 and were significantly associated with growth traits, and could be used as important candidate molecular markers for breeding selection of . Our results could provide an effective basis for the study of genetic improvement and selective breeding in

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杨月静,向梦斌,叶祥益,张争世,罗辉,叶华.齐口裂腹鱼SNP标记与生长性状的关联分析[J].中国水产科学,2018,25(2):278-285
YANG Yuejing, XIANG Mengbin, YE Xiangyi, ZHANG Zhengshi, LUO Hui, YE Hua. Association analysis between SNP markers and growth-related traits in Schizothorax prenanti[J]. Journal of Fishery Sciences of China,2018,25(2):278-285

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  • 在线发布日期: 2018-04-24
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