Abstract:In recent years, as fishery resources have dwindled, the hearing capacities of fish have been studied in ambient noise assessments, for the protection of fish, and also in the construction of marine ranching by using the acoustic domestication method. The use of an electrocardiogram (ECG) offers a method for the study of fish hearing capacities. The method comprises behavioral and electrophysiology sciences and is based on a conditioned reflex reaction to acoustic and electrical stimulus to monitor the hearing threshold through the heart rate of fish. , a bottom-living fish without a swim bladder, is predominantly a commercial species in northern China. The auditory sensor organs of are the otolith and the lateral line. The objective of this study was to use an ECG method to measure the hearing threshold of , by using self-established fish auditory sensation measuring devices, to provide a reference for the development and construction of marine ranching in China. The following acoustic stimulus frequencies were selected in the study:60 Hz, 100 Hz, 150 Hz, 200 Hz, 300 Hz, 400 Hz, 500 Hz, 700 Hz and used to measure the hearing threshold of 20 independent fish, from which audiograms could be drawn and analyzed. Before the experiment, an acoustic and electoral stimulus conditioned reflex in the fish was established to ensure the fish could develop memories of the stimulus, and then the hearing threshold was estimated by monitoring the heart rate of the fish, which was recorded by using a silver wire inserted in the fish heart. The ECG signals from 20 ind fish were obtained and the results were as follows:the hearing sensitive frequency bandwidth of was between 60 Hz and 200 Hz and the hearing threshold was below 136 dB (re:1 μPa); 100 Hz was the most sensitive frequency bandwidth and the hearing threshold was 94±1.5 dB. The advantages of ECG method were that a stable signal was obtained, the subjected fish show an obvious conditioned response to the acoustic stimulus, the quick recovery allowed repeated testing. The hearing threshold of , which have same body shape; they are sensitive to acoustic stimuli with a frequency of 60-200 Hz. The results of the study could help to promote fundamental research in fish auditory sensation and provide a reference for the construction of marine ranching and the future development of new tapes for fishing nets by using acoustic methods.