1. 中国水产科学研究院南海水产研究所, 农业农村部南海渔业资源开发利用重点实验室, 广东 广州 510300;
2. 上海海洋大学 水产与生命学院, 上海 201306
张楠(1987-),男,助理研究员,从事水产种质资源研究.E-mail:zhangnhs@163.com
S917
科技部科技基础性工作专项（2013FY110700）；中国水产科学研究院中央级公益性科研院所基本科研业务费专项（2013A11）；农业部重大财政专项（NFZX2013）；国家科技基础条件平台建设运行项目（2017DKA30470）.
WU Na
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural AffairsSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;
2. College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai 201306, China
以江门沿岸海域夏季采集到的鱼卵研究对象，利用DNA条形码技术分析鉴定鱼卵种类。本研究获得鱼卵个体有效线粒体COI序列信息217个，测序成功率为68.5%。经BOLD数据库比对分析，成功鉴定鱼卵5目14科19属20种（未知种2种）；种内遗传距离为0~0.006，平均遗传距离为0.002，种间遗传距离为0.149~0.325，平均遗传距离为0.255，种间遗传距离为种内遗传的128倍；鱼卵样品以鲈形目数量最多，占51.8%；鲱形目次之，占24.8%；其中鱼卵优势种为黄斑鲾（Megalaspis cordyla）、粗鳞鮻（Harpadon nehereus）、亚洲鱚（）。本次调查川山群岛东部海域获得鱼卵数量、鱼卵种类数均最多，是江门沿岸海域日本鳀、龙头鱼的主要产卵场；黄茅海、镇海湾西部水域均未获得鱼卵，可能与水域环境变化及陆源污染导致产卵环境破坏、亲体量减少有关。该研究结果揭示了江门沿岸海域夏季鱼卵分布格局，为其制定有效的产卵场保护措施提供依据，同时表明DNA条形码技术能有效地对鱼卵进行种类鉴定，可被广泛地应用于我国沿海海域鱼卵鉴定工作。
The main purpose of this study was to analyze and identify the species of fish eggs from the Jiangmen marine environment in summer, based on DNA barcoding technology. In the present study, we acquired a total of 217 effective mitochondrial COI sequences from fish eggs, and the success rate of sequencing was up to 68.5%. After the alignment of the BOLD database, we found that besides 2 species, a total of 5 orders, 14 families, 19 genera, and 20 species were identified. Statistical analyses showed that the interspecific genetic distances were 0~0.006, and mean interspecific genetic distance was 0.002, whereas the intraspecific genetic distances were 0.149~0.325, and mean intraspecific genetic distance was 0.255, which was 128-fold higher than the former. Among the fish eggs, the most abundant order was Perciformes (51.8%), followed by Clupeiformes (24.8%); the most abundant species was Megalaspis cordyla, , and . In this investigation, the most abundant fish eggs were found in eastern Chuanshan Archipelago, both in numbers and species. The Chuanshan Archipelago is also known as the main spawning site of . However, nearly no fish eggs were found in the Huangmao Bay area or western Zhenhai Bay, possibly owing to environmental disruption and a decrease of effective parent number, triggered by environmental variation and contamination from land-sourced pollutants. In general, we revealed the distribution pattern of fish eggs from the Jiangmen marine environment in summer, which provided a theoretical basis for developing effective protective measures for spawning sites. Meanwhile, the results also showed that DNA barcoding technology could be effective and broadly applied to identify the species of fish eggs in the marine habitats of our country.
张楠,吴娜,郭华阳,朱克诚,刘永,李纯厚,杨静文,江世贵,张殿昌.基于DNA条形码技术对江门沿岸海域夏季鱼卵的鉴定[J].中国水产科学,2018,25(4):721-727
ZHANG Nan, WU Na, GUO Huayang, ZHU Kecheng, LIU Yong, LI Chunhou, YANG Jingwen, JIANG Shigui, ZHANG Dianchang. DNA barcoding technology to identity spawn from the Jiangmen marine environment in summer[J]. Journal of Fishery Sciences of China,2018,25(4):721-727
