The Earth's biodiversity are closely altered by a variety of human activities at all scale from local to global. These changes have a strong potential effect to biological communities, ecosystem properties, social affairs and economic development they provide. China fishery is characterized by high biodiversity which cover 10 percent of the total earth species because its role geographical position that occupies the eastern part of the Eurasian continent adjacent to the western Pacific Ocean. In recent years, it is a very realistic and important scientific problem to protect and rationally utilize the biodiversity of fisheries globally. However, insufficient protection and hard utilization of fishery isn't a single case in China. To promote the development of fishery biological diversity in China, this paper puts forward the idea of DNA bar code methods for performing in fishery biology activities. It reviewed how DNA barcode expounds the sustainable development in the fishery biological resources, species identification and classification, fishery biodiversity monitoring, foreign species evaluation, market and supervision, fishery information and so on. Besides that, it further discussed the recent prospects for the DNA barcode in the biologic study of fishery organisms, water ecological research, electronic classification technology and the new technology of meta DNA barcoding. This research had appealed the continuous researches on construction of a fishery DNA barcode database and information platform of China which need to be constructed and shared nationally and globally.