1. 上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306;
2. 青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266071;
3. 中国水产科学研究院黄海水产研究所, 农业农村部海洋渔业可持续发展重点实验室, 山东 青岛 266071
孙东方(1991-),男,硕士研究生,主要从事三疣梭子蟹遗传育种工作.E-mail:961012207@qq.com
S917
国家虾蟹产业技术体系项目（CARS-48）；泰山领军人才工程高效生态农业创新类计划项目（LJNY2015002）；国家自然科学基金面上项目（41576147，41506186）；海洋渔业科学与食物产出过程功能实验室开放课题（2016LMFS-A12）；江苏省水产三新工程项目（Y2015-3）.
SUN Dongfang
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, ChinaLYU Jianjian
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, ChinaHUAN Pengpeng
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, ChinaGAO Baoquan
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, ChinaLIU Ping
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
3. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
本研究采用RACE技术克隆了三疣梭子蟹（NP）基因。该基因全长1920 bp，5'端非编码区237 bp，3'端非编码区1373 bp，开放阅读框309 bp，编码102个氨基酸，预测分子量10.8 kD，理论等电点7.42。Scylla paramamosain）NP4的同源性最高（89%），并且三疣梭子蟹与拟穴青蟹首先聚为一支。组织表达分析发现，NP基因在低盐胁迫过程中的表达规律发现，低盐胁迫可显著改变NP基因在脑、鳃和眼柄组织中的表达模式，整体呈现上调表达趋势，其中在脑、鳃和眼柄中表达量分别最高上调至7.7倍、2.8倍和2.6倍，且存在显著差异（<0.05）。本研究结果表明NP基因在三疣梭子蟹盐度适应中可能发挥一定作用且受神经内分泌系统的调控。
The swimming crab (), which is an economically important marine crab in China, is a widespread species in coastal Japan, Korea, and China. Neuropeptides have a significant effect on osmoregulation, growth, development, and immunity of crabs. To investigate the function of neuroparsin under low salinity stress in , the neuroparsin gene was cloned by rapid amplification of cDNA ends. The NP gene is 1920 bp long, including a 309 bp open reading frame (ORF) that encodes a 102-aa polypeptide; its isoelectric point was 7.42 and the molecular mass was 10.8 kDa. The NP gene contains 12 cysteine residues, which is a typical characteristic of neuroparsin in decapods. The homology and phylogenetic systematic analyses revealed that the highest homology and similarity (reaching up to 89%) occurred between P. trituberculatus clustered with . The tissue expression analysis showed that the expression of NP gene was relatively high in the brain, followed by the gill and eye, with very little or no expression in the ovaries, muscles, heart, liver, and pancreas. The expression pattern analysis of the NP gene under low salt stress condition showed that low salt stress can significantly change the expression of NP gene in the brain, gill, and eyestalk, and the overall expression was upregulated. In the brain, gill, and eyestalk, the expression of NP was 7.7, 2.8, and 2.6 times higher than that of the control, respectively (NP gene expression in the gills presented an increasing trend after the ablation of eyestalk. Furthermore, the expression of NP after the ablation of bilateral eyestalk was significantly higher than that after the ablation of unilateral eyestalk (NP gene might play a role in the salinity adaptation of , which is regulated by its neuroendocrine system.
孙东方,吕建建,环朋朋,高保全,刘萍.低盐胁迫下三疣梭子蟹蝗抗利尿肽基因的表达[J].中国水产科学,2018,25(5):967-975
SUN Dongfang, LYU Jianjian, HUAN Pengpeng, GAO Baoquan, LIU Ping. Expression analysis of neuroparsin gene under low salinity stress in swimming crab (Portunus trituberculatus)[J]. Journal of Fishery Sciences of China,2018,25(5):967-975
