摘要:潜在的毒性(0.05 mg/L)探讨臭氧对胚胎发育和初孵仔鱼的毒性作用。结果显示, 0.30 mg/L(0.30 mg/L)会造成胚胎油球数目增多、萎缩、解体和死亡, 0.05 mg/L也会导致初孵仔鱼的全部死亡。本研究结果旨在为石斑鱼育苗及养殖过程中正确使用臭氧消毒技术提供重要的数据参考和理论依据。

摘要:)通过生物学解剖对其消化道内容物中的藻类组成进行研究。结果显示, 2.51.5)和微小四角藻Tetraëdron minimum); 2.5

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摘要:Tilapia is one of the most extensively cultured species in aquaculture with great economic importance, and has been introduced to nearly 100 countries in the world. China is the biggest country in tilapia production, accounting for about 55% of the total production in the world. In which, Nile tilapia () has played an important role in developing tilapia industry. Since 1978, Nile tilapia first introduced to China, it has been multiply introduced from different countries or areas for several times. Consequently a number of domesticated strains or populations have been established and developed with different selective pressures and divergent genetic characteristics in China. This gives us a chance to combine multiple germplasm resources in breeding practices of tilapia. However, the genetic evaluation and investigation of these domesticated populations of Nile tilapia in China have been relatively poorly conducted. In this study, genetic diversity and genetic relationships of the eight domesticated populations of Nile tilapia in China were examined using partial mitochondrial control region (D-loop) sequence, including the Egypt (EGY), Gilada (GLD), American (USA), Luye (LY), Genomar (GNM), Baolu (BL), Guangdong (GD) and New GIFT (NGF) populations. The results showed that only 15 haplotypes were defined in the 237 analyzed individuals, in which, BL1 haplotype was shared by the five populations (BL, EGY, GLD, GNM, LY), but no one shared haplotype was found for all the populations. The Number of variable sites (), haplotype diversity () and 4 to 83, 0.50 to 37.26, 0.190 8 to 0.802 3 and0.000 8 to 0.056 9, respectively. The genetic diversity in the LY was the lowest in the eight populations analyzed. The results of analysis of molecular variance (AMOVA) indicated significant genetic divergence (<0.01) among the populations. The cluster analysis based on Kimura two-parameter model exhibited the two clusters among them, one cluster included the three populations of BL, GD and NGF, and another cluster consisted of the five populations of EGY, LY, GNM, GLD and USA. However, it was not well supported by the haplotype network profile in network. This study provided insight into the genetic basis of Nile tilapia in combining their germplasm in future

摘要:首先在凡纳滨对虾(Litopenaeus vannamei)转录组测序的基础上, 应用RT-PCR方法获得了凡纳滨对虾泛素交联酶E2 (UE2)基因的开放阅读框序列。该序列长为447 bp, 编码148个氨基酸, 理论相对分子量为16.84 kD, 等电点为4.90。同源性比对和系统进化分析显示, 不同物种的UE2基因具有较高的同源性, 其中凡纳滨对虾与中国明对虾(Fenneropenaeus chinensis)的同源性最高并聚为一支。半定量RT-PCR分析表明, UE2基因在所检测的组织中均有表达; 实时定量PCR分析表明, UE2基因在肝胰腺和肠中的表达量最高, 在心脏、鳃、胃和肌肉组织中的表达量略低, 在血淋巴中的表达量最低。原核表达分析结果显示, PCR®T7/NT-Topo TA-UE2表达载体在1.0 mmol/L IPTG、37℃诱导3 h条件下可获得纯度较高的分子量约17 kD的蛋白。利用亲和层析的方法将蛋白进行纯化用以制备抗体。研究结果将为深入研究UE2基因在对虾白斑综合症病毒侵染过程中的作用机制奠定基础。

摘要:大鲵虹彩病毒是近年中国大陆新发现的引起人工养殖大鲵Andrias davidianus大规模死亡的病毒病原。为了揭示大鲵虹彩病毒流行株的基因型差异本研究对年采集自全国不同大鲵养殖区域的患虹彩病毒病的大鲵样本进行了分子检测、病毒分离培养以及病毒主衣壳蛋白基因测序与比对分析。结果显示采自陕西、湖北、湖南、浙江、江西、福建等省的个样本检测为阳性通过细胞培养获得株病毒流行株。对该株流行株基因的测序与比对分析发现核苷酸序列相似性达到其推测的氨基酸序列无明显差异证实中国大鲵虹彩病毒流行株属同一基因型。所选大鲵虹彩病毒与蛙病毒分别聚为一枝但其亲缘关系较近。本研究结果旨为大鲵虹彩病毒病的疫苗研制及其免疫防控技术研究奠定基础。

摘要:、、不同组织之间甲基化水平差异显著。太平洋牡蛎中存在组织特异性甲基化片段和非组织特异性甲基化片段这可能与牡蛎较高的杂合度相关。对实验样品进行的荧光标记的扩增片段长度多态性基于以上结果可以推测基因组甲基化水平可能与个体遗传背景相关联。

摘要:年月、月下旬、月在黄河口海域进行的站位弓子网调查数据对该海域无脊椎动物群落组成、优势种、生物量、多样性、群落相似性等群落特征进行了研究。结果表明939种之间。纵肋织纹螺是黄河口无脊椎动物群落的主要优势种。调查期间生物量和丰度的变化趋势基本相似上升生物量最高值出现在丰度最高值出现在Shannon-Wiener月群落与月群落差异显著。根据研究结果与年代相比80距河口距河口断面无脊椎动物生物量和多样性在各航次间波动剧烈且规律性较差的无脊椎动物群落受黄河水沙输入影响更大

摘要:提高目的基因的转植效率与可遗传效率是转基因鱼研制的关键点之一。本研究利用近年开发的金鱼转座子系统进行转基因斑马鱼的研制将肌球蛋白轻链肌肉组织特异性表达红色荧光蛋白的转座子介导的转基因技术

摘要:(Andrias davidianus)通过细胞培养获得

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