Based on acoustic and bottom trawl survey data in the Guishan wind farm area in autumn 2019, we analyzed the species composition, species dynamics, and spatial distribution of fisheries resources in that area. A total of 72 species of swimming organisms and benthic invertebrates were caught, of which 34 species were assessed in this survey. The number and biomass estimated by the acoustic method were 5.97×10⁵ ind/km² and 15.13 t/km², respectively. Fish aggregation distribution characteristics were obvious in this area, and the density was higher in the northwest and southeast regions. The distribution range of fish single TS was -68--41dB, of which -68--58dB was dominant (79.12%), corresponding to a fish body length of 3-8 cm. With increasing water depth, the mean fish TS first decreased and then increased. This study was the first acoustic survey of fisheries resources in this area. The assessment results could provide accurate information on the quantity and distribution of various fish species, and provide basic data for monitoring long-term changes in fisheries resources after the wind farm has been completed.