三角帆蚌血淋巴细胞对插核刺激的响应及矿化功能
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作者单位:

1.上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306 ;2.上海海洋大学, 农业农村部淡水水产种质资源重点实验室, 上海 201306

作者简介:

孙钧林(2000-),男,硕士研究生,研究方向为贝类分子与细胞生物学.E-mail:tomasjl@163.com

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中图分类号:

S968

基金项目:

国家重点研发计划项目(2022YFD2400105); 国家现代农业产业技术体系(CARS-49); 国家自然科学基金项目(31872565).


Responses of hemolymphocytes to nucleus-inserting stimulation and biomineralization function of Hyriopsis cumingii
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Affiliation:

1.National Experimental Teaching Demonstration Center of Aquatic Science, Shanghai Ocean University, Shanghai 201306 , China ;2.Key Laboratory of Freshwater Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affars, ShanghaiOcean University, Shanghai 201306 , China

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    摘要:

    为深入研究淡水珍珠贝三角帆蚌(Hyriopsis cumingii)插核刺激对血淋巴细胞的影响及其在珍珠矿化中的功能, 本研究应用光学和电子显微镜, 观察了三角帆蚌血淋巴细胞的形态和分类特征, 并根据细胞复杂性、细胞大小、核质比将其分为 4 种类型。通过吉姆萨染色和流式细胞术分析发现, 插核刺激后血淋巴细胞的颗粒复杂度增加, 细胞直径变大, 颗粒细胞比例显著升高(P<0.05), 透明细胞比例显著降低(P<0.05), 同时通过组织切片观察发现, 插核部位附近有大量血淋巴细胞聚集且颗粒细胞在插核部位被招募。血淋巴细胞和血清中的 Ca2+含量在插核后 2 d、5 d 和 10 d 显著上升(P<0.05), 20 d 和 50 d 显著下降(P<0.05), 揭示了血淋巴细胞在数量组成、分布和 Ca2+ 水平上均响应插核育珠过程。通过体外碳酸钙结晶实验表明, 血淋巴细胞和血清能够影响方解石的形貌, 但未改变晶体晶型。综上所述, 血淋巴细胞对三角帆蚌插核刺激迅速响应且对生物矿化过程有调控作用, 本研究为进一步探究血淋巴细胞在贝类生物矿化中的调控作用奠定了理论基础。

    Abstract:

    In order to elucidate the effect of nucleus insertion stimulation on hemolymphocytes and their function in pearl mineralization of the freshwater pearl mussel Hyriopsis cumingii, the present study explored the morphology and classification of hemolymphocytes, including four types by means of light and electron microscopy based on cellular complexity, cell size, and nucleo-cytoplasmic ratio. Giemsa staining and flow cytometry analysis showed that the granularity complexity of hemolymphocytes increased significantly, cell diameter became larger after nucleus insertion, the proportion of granulocytes increased significantly (P<0.05), and the proportion of hyalinocyte decreased significantly (P<0.05). The results of histological observation showed that a large number of hemolymphocytes gathered near the insertion site and granulocytes were recruited. Ca2+ content in hemolymphocytes and serum continued to significantly increase 2, 5, and 10 d after insertion (P<0.05) and then significantly decreased 20 and 50 d after insertion (P<0.05), which revealed that the number composition, distribution, and Ca2+ level of hemolymphocytes all respond to nucleus insertion. In vitro calcium carbonate crystallization experiments showed that hemolymphocytes and serum were able to influence the morphology of calcite but did not change the crystal shape. In summary, hemolymphocytes respond rapidly to the stimulation of nucleus insertion and can regulate biomineralization. This study lays a theoretical foundation for further research on the regulatory role of hemolymphocytes in shellfish biomineralization.

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孙钧林,陈逸文,陈晓峰,李家乐,白志毅,李文娟.三角帆蚌血淋巴细胞对插核刺激的响应及矿化功能[J].中国水产科学,2025,32(5):622-634
SUN Junlin, CHEN Yiwen, CHEN Xiaofeng, LI Jiale, BAI Zhiyi, LI Wenjuan. Responses of hemolymphocytes to nucleus-inserting stimulation and biomineralization function of Hyriopsis cumingii[J]. Journal of Fishery Sciences of China,2025,32(5):622-634

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  • 收稿日期:2024-12-22
  • 最后修改日期:2025-01-16
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  • 在线发布日期: 2025-08-04
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