Tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-primer ARMA-PCR) was introduced to investigate single nucleotide polymorphisms (SNPs) genotyping in Chinese shrimp () and 80 were studied. Twenty out of the 80 SNP tetra-primer ARMA-PCR primer sets were validated following touchdown profiles and the outer and the expected inner bands were amplified. Homozygous and heterozygous were detected by agarose gel and the genotypes were obtained. Polymorphism of these distinct loci was assessed using 30 individuals, and the results showed that the 20 loci were all polymorphic. The values of and PIC varied from 1.127 to 1.993, from 0.136 to 0.607, from 0.119 to 0.492 and from 0.145 to 0.3730, respectively. The results indicated that tetra-primer ARMA-PCR is a simple, rapid and efficient method for SNP genotyping which make it useful in a broad aspects of genetic and breeding studies.