Cytochrome P450s (CYPs) widely distribute in the liver of aquatic organisms and play a key role in drug metabolism. CYPs catalyze the N-deethylation of (CIP). However, there is limited information regarding the action of CYPs in fish. We evaluated the of baicalin (100 mg/kg). We measured the enzymatic activity of cytochrome P450 1A (CYP1A) and 3A (CYP3A) in liver microsomes. In addition, we documented the pharmacokinetics of decreased significantly over time and both CL inceased after oral administrated of elimination was accelerated. The was 1.48% and 2.22% for the BL and GZ groups, respectively (control: 0.95%). The AUC_{was 2.16% and 1.76% for the BL and GZ groups, respectively (control: 1.7%). Thus, BL and GZ contributed to induce N-deethylation of . Pretreatment with significant erythromycin-N-demethylation (ERND) activity, the specific probes for CYP1A and CYP3A, BL and GZ accelerated the elimination of metabolite was related to the induction of CYP1A and CYP3A enzymatic activity.}