草鱼胰岛素样生长因子1受体基因cDNA全序列的克隆及功能

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草鱼胰岛素样生长因子1受体基因cDNA全序列的克隆及功能
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作者单位:上海海洋大学 农业部淡水水产种质资源重点实验室, 上海 201306
作者简介:周春雪(1987-), 女, 硕士, 主要从事鱼类遗传育种研究. E-mail: xiangxiaoxuehua@sina.com
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中图分类号:S917
基金项目:“十二五”国家863计划主题项目(2011AA100403); 农业部公益性行业科研专项(201203081, 200903045); 上海高校知识服务平台(ZF1206).

cDNA cloning and function of insulin-like growth factor-I receptor gene (Igf-Ir) in grass carp (Ctenopharyngodon idellus)

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Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China

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序列全长端非翻译区581 bp, 该基因编码的氨基酸序列与鲤)IGF-93%, 16 hpf(hours post fertilization)在成鱼大部分组织中均有表达。原位杂交结果显示 mRNA其中在脑部、脊索和尾部等生长旺盛组织的细胞中表达量较高。基因在生长发育信号通路中的作用和育种提供了基础资料。

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The insulin-like growth factor-I receptor (IGF-R) is an important component of the insulin-like growth factor system, which plays a key role in embryonic growth, formation of the nervous system, skeletal muscle development, and also appears to contribute to the establishment and progression of tumors. Despite its importance, little is known about the tissue distribution and expression pattern of during embryonic development in most commercially important cultured fish species. We isolated a 5 741 bp full-length cDNA from grass carp( cDNA included an 822 bp complete 5′ untranslated region (5′UTR), a 581 bp 3′UTR, and a 4 338 bp open reading frame (ORF) region which encoded a 1 445 amino acid protein. The mature peptide contained seven structural domains including two Recep-L-domains, one Furin-like domain, three FN3 domains, and one PTKc-IGF-1R domain. Among these, the PTKc-IGF-1R domain was the most conservative domain of IGF-R may play different transductional roles among different teleost species. The ORF deduced from the cDNA sequence was 95%, 93%, and 66% identical to the corresponding regions of IGF-R, respectively, indicating a high level of conservation of IGF-R has a high amino acid sequence identity with common carp and zebrafish IGF-IGF- mRNA was detected during embryogenesis, beginning at 16 hpf (hours post fertilization) and continuing through the larval stages. The mRNA was transcribed in almost all adult tissues except the intestine, with expression being highest in the grass carp heart. Using whole mount mRNA was detected ubiquitously in 16 hpf embryos, with strong signals in the brain and spinal cord, but only a weak signal in the tail-fin. In 24 hpf embryos, the mRNA signal was strong in brain, spinal cord, and in rapidly growing regions such as tail-fin tip. Our results provide insight into the IGF-R signaling pathways and provide a basis for future genetic breeding of grass carp.

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周春雪,蒋霞云,陈杰,邹曙明.草鱼胰岛素样生长因子1受体基因cDNA全序列的克隆及功能[J].中国水产科学,2014,21(3):442-453
ZHOU Chunxue, JIANG Xiayun, CHEN Jie, ZOU Shuming. cDNA cloning and function of insulin-like growth factor-I receptor gene (Igf-Ir) in grass carp (Ctenopharyngodon idellus) [J]. Journal of Fishery Sciences of China,2014,21(3):442-453

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在线发布日期: 2015-07-17
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