The (cholecystokinin) gene is a peptide substance which mainly distributed in the brain and intestine in a variety of macromolecules. Cholecystokinin plays an important role in the functions of regulating food intake and gastrointestinal motility, promoting pancreatic secretion and gallbladder contraction, etc. However, there are differences in the regulation modes among different species. In order to study the appetite regulation of CCK in . The cDNA full-length of the cholecystokinin gene from was cloned using RACE and RT-PCR methods, and it belonged to the CCK-1 subtype by bioinformatics analysis. The mRNA levels in major tissues, before and after feeding, fasting and refeeding were detected and analyzed using quantitative real-time PCR. The results showed that the full-length cDNA of gene was 773 bp, the open reading frame (ORF) was 372 bp and can encode 123 amino acids. The CCK was composed of a signal peptide and a conserved domain of the typical CCK-8 peptide, which was a hydrophilic protein but had no transmembrane structure. Sequence homologous analysis of amino acids showed that the CCK of had high homology with those of the fish belonging to Cypriniformes-teleosts, and low homology with mammals and amphibians. Tissue distribution analysis showed that the expression of was highest in brain and was high in intestines, heart, liver, spleen, kidney, skin, gill, eyes, maw, while it was lowest in muscle. The mRNA expression was highly elevated after feeding, and decreased after fasting and re-increased after refeeding. The results indicated that the gene was both the postprandial satiety signal and the long-term appetite regulation factor in .